萝卜自交不亲和特性的荧光快速鉴定  

Rapid identification of self-incompatibility in radish(Raphanus sativus L.) with fluorescent observation

作　　者：赵天荣[1] 龚义勤[1] 柳李旺[1] 黄丹琼[1] 周艳孔[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/园艺学院,江苏南京210095

出　　处：《南京农业大学学报》2007年第4期30-34,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30300238;30571193);江苏省自然科学基金项目(创新人才BK2004418);江苏省高技术研究计划项目(BG2005314);江苏省十字花科蔬菜种质资源基因库建设项目(sx(2007)913)

摘　　要：以萝卜高代自交系为材料,利用荧光显微镜观察其花期和蕾期自花授粉的柱头与花柱,结果发现自交不亲和与自交亲和材料花粉萌发和花粉管伸长状况存在明显差异。自交不亲和材料花期授粉后柱头上出现严重的胼胝质反应,花粉很少萌发,即使萌发也不能正常生长,出现弯曲或背向生长,花粉管顶端膨大不能穿越柱头乳突细胞;自交亲和系萝卜单株花期、蕾期以及自交不亲和系单株蕾期授粉后花粉多数能够正常萌发并穿越柱头进入子房。对供试材料进行了田间自交亲和指数测定,结果与荧光显微镜测定法鉴定结果高度一致。因此利用荧光显微镜观察法可以准确快速鉴定萝卜的自交不亲和性,从而提高优良自交不亲和系选育效率。Using the advanced inbred lines as the materials, the interaction of pollen-stigma was investigated after self-pollination both in the bud and at anthesis with the fluoroscope in radish. The results indicated that there were obvious differences in the germination of pollen grains in stigmas and growth of pollen tubes in styles between the self-incompatible and self-compatible lines. Few pollen grains of self-pollination at anthesis attached and germinated in the stigma of self-incompatible individuals, and intensive callose reaction occurred on the surface of stigma. The germinated pollen grains could not grow normally and fail to penetrate into the stigma with the distorting of pollen tubes, swelling of the tops and failure to further growth. Most pollen grains of self-pollination both in the bud and at anthesis germinated naturally and penetrated into the stigmas and finally succeeded in reaching to the ovary in self-compatible individuals, and theses also occurred in the bud self-pollination in self-incompatible individuals. The self-incompatibility （SI） indexes of all the lines were tested with the traditional index method, and it was high accordant with the results from the fluoroscope observation, indicating that self-incompatibility of radish lines can be identified with the fluoroscope observation method. Therefore, fluoroscope observation method is a good efficiently tool for development of elite self-incompatible lines in radish breeding program.

关 键 词：萝卜 自交不亲和性 荧光显微测定 

分 类 号：S631.1[农业科学—蔬菜学]