西班牙根结线虫rDNA特性及其检测方法  

Characterization of rDNA and detection of Meloidogyne hispanica

作　　者：胡永坚[1] 王暄[1] 李红梅[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2007年第4期62-65,共4页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30471141);国家863计划项目(2001AA249021)

摘　　要：通过对rDNA的ITS区和IGS2区进行PCR扩增并对产物测序,比较分析了西班牙根结线虫与中国4种常见的南方根结线虫、爪哇根结线虫、花生根结线虫和北方根结线虫群体rDNA的ITS区和IGS2区的序列差异,选择限制性内切酶TaqⅠ对IGS2区的PCR扩增产物进行酶切,获得了西班牙根结线虫的554 bp的特异性条带。表明用此方法可以明显将西班牙根结线虫与其他4种常见的根结线虫群体区分开,其灵敏度可达到单条根结线虫二龄幼虫。The internal transcribed spacers （ITS） and intergenic spacer 2 （IGS2） of rDNA were amplified and the PCR products were sequenced from populations of Meloidogyne hispanica. Based on the differences between the sequences of M. hispanica, M. incognita, M. javanica, M. arenaria, and M. hapla, RFLP was carried out on the PCR products of IGS2 region by Taq I , and a specialized 554 bp fragment from M. hispanica can distinguish it from the other four Meloildogyne species. Reliability and sensitivity of IGS2-RFLP can be achieved from the single juvenile.

关 键 词：西班牙根结线虫 RDNA ITS IGS2 RFLP 

分 类 号：S432.45[农业科学—植物病理学]