蚯蚓纤溶酶的糖成分分析  

Composition Analysis of Sugar of the Earthworm Fibrinolytic Enzyme

作　　者：张伟[1] 刘秀丽[1] 赵晓瑜[1] 

机构地区：[1]河北大学生物技术研究中心

出　　处：《生物技术》1997年第4期19-22,共4页Biotechnology

摘　　要：以大豆胰蛋白酶抑制剂为配基的亲和层析制备的蚯蚓纤溶酶是一组糖蛋白。通过苯酚一硫酸法测中性糖，3，5一二硝基水扬酸法测还原糖，硅胶G薄层层析法鉴定糖的种类，结果为：蚯蚓纤溶酶主要含有中性己糖，含量为15％左右，TFMS的去糖处理会使蚯蚓纤溶酶酶活丢失．The earthworm fibrinolytic enzyme was prapared by affinity chromatography using soy been trypsin inhibitor as a ligand. It was a set of glycoproteins. The protocols used were phenol sulfuric acid method. 3, 5 -dinitrosalicylic acid method and thin-layer chromatography of silic gel G.1 was shown that the suger the enzyme contained was about 15% and mainly neutrol hexose.The enzyme was lost its activty after sugar being deleted with TFMS.

关 键 词：蚯蚓纤溶酶 药物 糖成分 

分 类 号：R977.3[医药卫生—药品]