复方洗必泰漱口液质量分析方法的建立及抑菌作用  

Quality analysis and bacteriostatic evaluation on compound chlorhexidine gargle in vitro

作　　者：罗东[1] 冉光炳[1] 戴青[1] 何旭露[1] 刘晶[1] 

机构地区：[1]第三军医大学西南医院药学部,重庆400038

出　　处：《第三军医大学学报》2007年第3期264-266,共3页Journal of Third Military Medical University

摘　　要：目的建立复方洗必泰漱口液质量分析方法,并考察其对口腔中常见需氧菌的体外抑菌作用。方法采用高效液相色谱法（high-performance liquid chromatography,HPLC）测定复方洗必泰漱口液中三主药的含量；采用琼脂平板二倍稀释法测定该制剂最低抑菌浓度（MIC）。结果醋酸洗必泰、盐酸达克罗宁、地塞米松的浓度分别在8-40、10-50、10-50μg/ml范围内呈良好的线性关系,相关系数r分别为0.9992、0.9994和0.9999；体外抑菌试验显示复方洗必泰漱口液对金黄色葡萄球菌,表皮葡萄球菌,铜绿假单胞菌及大肠埃希菌具有良好的抑菌作用。结论高效液相色谱法测定制剂中三主药含量的方法可靠,复方洗必泰漱口液对口腔常见需氧菌的体外抑菌作用试验表明其为-疗效满意的口腔制剂。Objective To establish a quality analysis method for the compound chlorhexidine gargle, and study its inhibitory effect of anti-oral aerobic bacteria in vitro. Methods The concentrations of 3 ingredients in the compound chlorhexidine gargle were determined by high-performance liquid chromatography （HPLC）, S. aureus, S. epidermidis,Ps, aeruginosa, and E. coli were chosen as the experimental bacteria. Minimal inhibi- tory concentration （MIC） was measured by agar dilution test. Results A good linear relationship was observed when chlorhexidine acetate, dyclonine hydrochloride and dexamethasone were respectively in a range of 8-40, 10-50, and 10-50 μg/ml. Their correlation coefficients were 0.9992, 0.9994 and 0.9999 respec- tively. The compound chlorhexidine gargle showed better bacteriostatic effects on above-mentioned bacteria. Conclusion The compound chlorhexidine gargle was a better oral preparation and might be an effective agent in treating S. aureus, S. epidermidis,Ps, aeruginosa, and E. coli caused diseases.

关 键 词：醋酸洗必泰 盐酸达克罗宁 地塞米松 质量控制 抑菌作用 

分 类 号：R927.2[医药卫生—药学] R965