用于禽流感H5、H9亚型同步检测的压电免疫传感器阵列的研制  

Research on the Piezoelectric Immunosensor Array for the Simultaneous Determination of the H5, H9 Subtype Avian Influenza Viruses(AIV)

作　　者：刘毅新[1] 王弘[1] 刘仲明[2] 孙远明[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]广州军区广州总医院,广东广州510010

出　　处：《中国医学物理学杂志》2007年第6期425-427,434,共4页Chinese Journal of Medical Physics

基　　金：广东省重大科技专项资助(No.2005A2090101)

摘　　要：目的:研制禽流感H5、H9亚型同步检测的压电免疫蛋白芯片。方法:采用聚乙烯亚胺(PEI)粘附、戊二醛(Glu)交联的方法,将禽流感H5、H9抗原固定于石英晶体的金电极表面。探讨了抗原的最佳固定条件,研究了压电蛋白芯片对阳性、阴性标准血清的响应情况。结果:该芯片用于检测浓度范围分别为0.123mg/mL～0.615mg/mL、0.123mg/mL～1.23mg/mL的禽流感H5、H9标准血清时,呈较好的线性关系,相关系数分别为0.9585、0.9854。结论:阳性标准血清的响应值与阴性标准血清的噪声值之比大于2,可用于定性定量检测。Objective： The purpose of this experiment was to research the piezoelectric immunity protein chips for the detection of H5 ,H9 subtype AIV. These two subtypes of avian influenza viruses antigen was fixed on gold electrode surface of the quartz crystal by polyethylenimine adhesion and glutaraldehyde cross-linking. Methods： The optimum condition of the immobility of antigen and the response value of piezoelectric immunity protein chips to the positive and negative standard serum was researched. Results： The resonant range of the sensor is between 0.123 mg/mL-0.615 mg/mL and 0.123 mg/mL- 1.23 mg/mL and the correlated coefficient is 0.9585 and 0.9854. Conclusion： The sensitivity for positive serum was 2 times higher than negative serum, which can be used to qualitative and quantitative detection for H5 and H9 subtype AIV.

关 键 词：禽流感 同步检测 免疫传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]