MAN2C1基因在病毒感染中的作用  

The Function of MAN2C1 Gene on Virus Infection in Transgenic Mice

作　　者：刘红霞[1] 朱华[1] 代小伟[1] 朱立平[2] 秦川[1] 张连峰[1] 

机构地区：[1]中国医学科学院实验动物研究所北京协和医科大学比较医学中心,北京100021 [2]中国医学科学院基础医学研究所,北京100005

出　　处：《中国比较医学杂志》2007年第11期621-624,I0002,共5页Chinese Journal of Comparative Medicine

基　　金：北京市转基因平台建设项目TC2006-02(Z0006303041231);中央级公益性科研院所基本科研业务费专项基金(DW200704)

摘　　要：目的通过观察MAN2C1转基因小鼠对H5N1高致病性禽流感病毒的易感性,以了解此转基因小鼠的免疫应答情况和MAN2C1基因在病毒感染中的作用。方法以H5N1高致病性禽流感病毒滴鼻感染MAN2C1转基因小鼠,HE染色观察小鼠肺组织病理变化;RT-PCR和免疫组织化学方法检测小鼠肺组织H5N1病毒载量;间接ELISA方法检测小鼠血清抗体滴度变化。结果与对照组小鼠比较,MAN2C1转基因小鼠表现为更为严重的间质性肺炎,肺组织病毒载量增加,外周血中性粒细胞数目降低,淋巴细胞数量增加,血清IgG抗体滴度降低。结论MAN2C1基因抑制了小鼠的体液免疫。Objective To study the immunologic function of MAN2C1 gene in the transgenic mice. Methods Infection of transgenic mice with H5N1 were performed by nasal dripping. The pathological changes of lung tissues were observed by HE staining and immuno-histochemistry. H5N1 viral load in lung of mice were detected with RT-PCR. Serum antibody against H5N1 was measured with indirect ELISA. Results The pathological changes were more serious in the transgenic lung than that of control mice. The expression levels of H5N1 virus antigen and virus load in the lung tissues of transgenic mice were obviously higher than that in control mice. A decreased level of serum antibody against H5N1 was also observed in the transgenic mice compared with control mice. Conclusion Our results suggest that MAN2C1 gene down-regulates the humoral immunity against virus infection.

关 键 词：MAN2C1 转基因 免疫组化 

分 类 号：R392[医药卫生—免疫学]