HBV整合引起HepG2细胞microRNA表达谱变化分析  

Differential microRNA expression profile of HepG2 cells induced by HBV integration

作　　者：韩俊峰[1] 阮志华[2] 张蓓[1] 吴玉章[1] 

机构地区：[1]第三军医大学基础医学部全军免疫学研究所,重庆400038 [2]第三军医大学西南医院肿瘤科,重庆400038

出　　处：《第三军医大学学报》2007年第22期2128-2130,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金青年基金(30400385)~~

摘　　要：目的分析HBV整合后引起的肝细胞microRNA表达谱的变化情况。方法利用microRNA芯片,以稳定表达HBV的HepG2.2.15细胞为HBV感染模型,以HepG2细胞为对照,分析二者microRNA表达谱的异同,得到因HBV感染而产生变化的microRNA;并用定量PCR技术来验证结果的可靠性。结果通过microRNA芯片分析和定量PCR验证,HBV感染后可使hsa-miR-30a-5p、hsa-miR-24、hsa-let-7a、hsa-let-7c、hsa-let-7f和hsa-miR-23b6种microRNA下调,使hsa-miR-194、hsa-miR-200a和hsa-miR-3453种microRNA上调。结论HBV整合后,可引起肝脏细胞microRNA表达谱变化。Objective To investigate the differential expressions of microRNA after HBV integration. Methods A human microRNA microarray was used to analyze the microRNA expression profile in HepG2.2.15 cell line, a novel model of HBV infection. HepG2 cells served as a mock control. Then the microRNA with differential expression were proved using real-time PCR. Results HBV infection induced 6 microRNA down-regulated （hsa-miR-30a-5p, hsa-miR-24, hsa-let-7a, hsa-let-7c, hsa-let-7f and hsa-miR-23b） and 3 microRNA up-regulated （hsa-miR-194, hsa-miR-200a and hsa-miR-345）. Conclusion HBV integration can induce the changes of microRNA expression profile in hepatocytes.

关 键 词：microRNA芯片 HBV MICRORNA 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学] R394.3[医药卫生—基础医学]