HCN4基因修饰大鼠MSCs体外诱导获得的心脏起搏样细胞动作电位检测  

Action potential of cardiac pacemaker cells differentiated from mouse mesenchymal stem cells after HCN4 gene modification

作　　者：王泽文[1] 宋治远[1] 姚青[1] 

机构地区：[1]第三军医大学西南医院心血管内科

出　　处：《第三军医大学学报》2007年第22期2139-2141,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30370585)~~

摘　　要：目的探讨超极化激活及环化核苷酸调控的阳离子通道亚型4(hyperpolarization-activated cyclic nucleotidegated cation channel4,HCN4)基因修饰大鼠MSCs体外诱导分化的心脏起搏样细胞的动作电位特性。方法以HCN4基因修饰大鼠MSCs体外诱导获得的具有自发搏动的心脏起搏样细胞为实验组,同期培养的原代乳鼠窦房结细胞为对照组,采用全细胞膜片钳技术对两组细胞的动作电位进行检测。结果心脏起搏样细胞及原代培养乳鼠窦房结细胞均可记录到具有舒张期自动去极化的动作电位,心脏起搏样细胞的动作电位特性与原代培养的乳鼠窦房结细胞相比,其静息电位、动作电位幅度及动作电位周期均无显著性差异(P>0.05),但阈电位较原代培养的乳窦鼠房结细胞显著降低(P<0.01)。结论从电生理角度证实,心脏起搏样细胞的特性与原代培养的乳鼠窦房结细胞相似。Objective To investigate the potassium currents of the cardiac pacemaking cells induced and differentiated from rat mesenchymal stem cells （MSCs） modified by HCN4 gene. Methods Identified cardiac pacemaking cells were adopted as the experiment group, and the sinoatrial node cells of original infant rat cultured in the same period were regarded as the control group. Whole cell patch was used to measure the action potential of the pacemaking cells and sinoatrial node cells. Results Action potential of automatic depolarization at dilatation was recorded in both the differentiated cardiac pacemaking cells and sinoatrial node cells. There was no significant difference on amplitudes of resting potential, amplitudes and cycle of action potential [ （ -50 ±2.8） vs （ -55 ±5.5） ,（ -60 ±2.5） vs （ -65 ±2.5） ,（240±57） ms vs （250 ±60） ms] , but the field potential was much lower in cardiac pacemaking cells than the control group [ （ - 30 ± 2.5 ） vs （ - 55 ± 5.5 ） ,P 〈0.01 ]. Conclusion Whole cell patch technology can record the action potential of automatic depolarization at dilatation in the cardiac pacemaking cells, with the similar characteristics of the sinoatrial node cells of infant rat cultured originally.

关 键 词：心脏起搏样细胞 动作电位 全细胞膜片钳 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R329.27[医药卫生—基础医学]