TGF-β_1在ConA激活的CD4^+T细胞抑制NK细胞杀伤毒性中的影响  

Cytotoxicity of NK cells inhibited by CD4^+ T cells stimulated by ConA

作　　者：王庆红[1] 王晓娟[1] 甘成军[1] 彭旭[1] 罗高兴[1] 吴军[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所

出　　处：《第三军医大学学报》2007年第22期2145-2147,共3页Journal of Third Military Medical University

基　　金：重庆市科委自然科学基金(2006BB5071)~~

摘　　要：目的初步探讨ConA激活的CD4+T细胞对NK细胞杀伤毒性的影响及TGF-β1分子在其中的作用。方法磁珠分选纯化BALB/c小鼠脾脏CD4+T细胞与NK细胞,体外用ConA激活CD4+T细胞,将激活的CD4+T细胞与NK细胞共培养,并加入TGF-β1抗体,共培养24、48、72h和96h后,用51Cr标记的YAC-1检测NK细胞的杀伤毒性。结果ConA激活的CD4+T细胞能够在所测的4个时相点显著抑制NK细胞的杀伤毒性,抑制率分别为25.00%、31.60%、34.70%和46.50%;在此共培养体系中加入TGF-β1抗体后,抑制率分别增加到63.80%、59.43%、60.70%和90.90%。结论ConA激活的CD4+T细胞能够显著抑制NK细胞的杀伤毒性,并且这种抑制作用随着TGF-β1抗体的加入而增强,表明TGF-β1分子影响了ConA激活的CD4+T细胞对NK细胞杀伤毒性的抑制。Objective To explore the effect of CD4^＋ T cells stimulated by ConA on the cytotoxic activity of NK cells. Methods CD4^＋T cells and NK cells were purified from spleen of BALB/c mice spleen by magnetic cell sorting. CD4^＋T cells stimulated by ConA and NK cells were co-cultured with or without anti-TGF-β1 antibody in the co-culture system. The cytotoxicity of NK cells was assayed by YAC-1 labeled by ^51Cr in 24, 48, 72 and 96 h. Results The cytotoxicity of NK cells were inhibited significantly by CD4^＋T cells activated by ConA at 4 time points and the inhibitory rate respectively was 25% , 31.6% , 34.7% and 46.5%. After antiTGF-β1 antibody was added into the co-culture system, the inhibitory rate reached to 63.8% , 59.43% , 60. 7% and 90.9%. Conclusion In vitro, CD4^＋T cells stimulated by ConA notably inhibits the cytotoxicity of NK cells, and TGF-β1 pathway may be involved in this action.

关 键 词：CD4^+T细胞 NK细胞 TGF-Β1抗体 细胞毒性 

分 类 号：R392-33[医药卫生—免疫学] R392.12[医药卫生—基础医学]