甘菊DlBADH1基因启动子DBP12表达特性研究  

Expression Pattern of DBP12 Promoter of DlBADH1 Gene from Dendran the ma lavandulifolium

作　　者：曹华雯[1] 刘振林[1] 夏新莉[2] 尹伟伦[2] 戴思兰[1] 

机构地区：[1]北京林业大学园林学院,北京100083 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《分子植物育种》2007年第6期758-764,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(No.30471419)

摘　　要：用pCAMBIA1305.2做载体,构建含有甘菊(Dendranthema lavandulifolium)DlBADH1基因(登录号:DQ011151)启动子DBP12(登录号:DQ497621)的质粒pCHW-2。利用叶盘转化法,通过农杆菌EHA105菌株介导,将重组质粒导入烟草叶片。采用GUS组织活性检测法鉴定烟草转基因植株,结果表明:DPB12启动子驱动的GUS基因表达无组织特异性。对转基因植株进行NaCl、ABA、SA处理,GUS荧光测定发现:100μmol/L ABA胁迫处理24 h和400 mmol/L NaCl胁迫处理48 h,转pCHW-2烟草植株叶片中GUS酶活性均有大幅度提高。该结果表明:DlBADH1基因启动子DBPl2是一个诱导型启动子。这一研究结果为进一步研究甘菊中BADH基因调控表达机理提供了参考。The construct pCHW-2 consisting of DBP12 promoter （Genbank Accession No： DQ497621） of DlBADH1 （Genbank Accession No： DQ011151） gene from Dendranthema lavandulifolium L. genome was transferred into tobacco plants via Agrobacterium tumefaciens EHA105. The regenerated plants were analyzed by GUS histo-chemical staining. The results showed that the expression of GUS gene under the control of DBP12 promoter was no tissue-specific. Under the treatments of NaCl, ABA and SA stress, the expression efficiency of the promoter in transgenic plants were analyzed by GUS activity assay. The level of GUS activity in the leaves of transgenic tobacco plants increased steadily after 24 h exposure to 100 μmol/L ABA and 48 h exporsure to 400 mmol/L NaCl, which could imply that DBP12 promoter of DlBADH1 gene should be an inducible promoter. The results might provide a reference for further studies on expression pattern of BADH gene in Dendranthema lavandulifolium.

关 键 词：DBP12启动子 转基因烟草 ABA SA NACL GUS活性 

分 类 号：Q943[生物学—植物学]