分离培养子宫内膜腺上皮和间质细胞方法的改进  

Improvement on isolation and culture of human endometrial glandular epithelial cells and stromal cells in vitro

作　　者：王佳[1] 姚珍薇[1] 唐良萏[1] 钟梁[2] 令狐华[1] 谯建[1] 刘北忠[2] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016 [2]重庆医科大学检验系,重庆400016

出　　处：《重庆医科大学学报》2007年第12期1276-1278,1346,共4页Journal of Chongqing Medical University

摘　　要：目的：探索一种获得高产量、高纯度子宫内膜腺上皮细胞和间质细胞的分离培养方法。方法：通过高浓度胶原酶和DNase联合消化、过滤及贴壁纯化等技术，分离培养30例子宫内膜腺上皮和间质细胞，并拟传代。结果：29例标本培养成功，每1g子宫内膜组织约可分别得到（40～50）×10^6个腺上皮细胞和（40～50）×10^6个间质细胞，细胞产量高。并且腺上皮细胞纯度率约96％，间质细胞纯度率达98％以上。间质细胞可以有限传代，腺上皮细胞不能传代。结论：通过改良步骤，可提高腺上皮和间质细胞产量，同时保证了其纯度及活性，不失为一种较理想的子宫内膜细胞分离培养方法。Objective：To explore the isolation and culture of a better yield of purified human endometrial glandular epithelial cells and stromal cells. Methods：Digestion with high concentrated collagenase and DNase,filtration and adhesion purification were used to isolate,purify human endometrial glandular epithelial cells and stromal cells in 30samples. And the two kinds of cells were reproduced. Results：The endometrial cell culture was successfully established in 29 of 30 endometrium samples. From one gramme of endometrial tissue,the yields of glandular epithelial cells and stromal cells were （40～50） × 10^6 and （40～50） × 10^6 respectively. The purities of glandular epithelial cells and stromal cells were 96% and 98% respectively. Stromal cells could be reproduced into several passage. However,glandular epithelial cells could not be reproduced. Conclusion：A better yield of purified human endometrial glandular epithelial cells and stromal cells can be obtained by the modified culture procedure.

关 键 词：子宫内膜 腺上皮细胞 间质细胞 细胞培养 

分 类 号：R711.74[医药卫生—妇产科学]