一株新的木素过氧化物酶产生菌的分离和鉴定  

Separation and Identification of One New Lignin Peroxidase-producing Trametes Strain

作　　者：袁璟[1] 洪宇植[1] 张小青[2] 肖亚中[1] 

机构地区：[1]安徽大学生命科学学院和现代实验技术中心,安徽合肥230039 [2]中国科学院微生物研究所,北京100080

出　　处：《生物技术通讯》2007年第6期949-951,共3页Letters in Biotechnology

基　　金：国家自然科学基金项目(30470056;30670069);安徽省优秀青年科技基金项目(04043048);安徽省教育厅自然科学基金项目(2006KJ048A);安徽大学211工程学术创新团队资助项目(02203109)

摘　　要：目的:木素过氧化物酶在工业和环保方面具有潜在应用价值。从腐木上分离得到一株新的木素过氧化物酶合成菌,该菌株具有香栓菌(Trametes suaveolens)的形态特征,需要对其进一步确认。方法:用rDNA ITS序列的分子分类方法对该菌株进行鉴定。结果:PCR得到的ITS片段长621 bp,其序列与一株香栓菌具有最高的相似性(97.6%),并明显不与栓菌其他种聚类。结论:该分离种为一株新的香栓菌,命名为T.suaveolens 1523。Objective： Lignin peroxidase possesses potential in many applications for industrial and environmental purposes. A new lignin peroxidase-producing fungus strain was separated from rotten wood. The isolate has the basic characteristics of Trametes suaveolens（L.） Fr. in morphology, which needs to be confirmed. Methods： Molecular taxonomy based on internal transcribed spacer （ITS） sequence of ribosome DNA was further performed to identify the isolate. Results： The 621 bp DNA sequence obtained by PCR was most similar to the ITS sequence of a T.suaveolens strain with a 97.6% similarity, and was obviously separated from those of other Trametes species. Conclusion： This isolate was identified as a new strain of T.suaveolens, designated as T.suaveolens 1523.

关 键 词：ITS序列 木素过氧化物酶 分子分类法 香栓菌 

分 类 号：Q93-331[生物学—微生物学]