丙型肝炎病毒体外培养系统研究进展  

Advances of Culture System In Vitro of Hepatitis C Virus

作　　者：高丽[1] 夏雪山[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650224

出　　处：《生物技术通讯》2007年第6期1010-1012,共3页Letters in Biotechnology

基　　金：国家自然科学基金项目(30460119);云南省国际合作项目(2006GH07)

摘　　要：由于缺少丙型肝炎病毒(HCV)的细胞培养系统和小动物模型,因此对其生活周期、作用机制至今仍不是很清楚,从而严重阻碍了丙型肝炎疫苗及相关治疗药物的开发与研制。一直以来,人们研究的HCV体外培养细胞模型包括感染模型和转染模型两种,感染模型由于原代肝细胞培养问题未能解决而难以成功,而转染模型的发展可喜。但是HCV复制子只能在极少数细胞中短暂复制,且产生的病毒量很低。1999年建立了亚基因组复制子,使人们有机会对其进行深入研究,但须人为引入碱基突变。最近建立的全基因复制子不需要引入突变即形成病毒粒子,是一项重大突破。概述了HCV体外培养系统的研究进展。On account of absence of cell culture system and small animal model, the life cycle and functionary mechanism of hepatitis C virus（HCV） are still poorly understood. The development of vaccine and associated antiviral drugs are deeply hampered. Now, the cell model of HCV culturing in vitro is divided into infective and transfective model. Because the culture of primary hepatocytes isn＇t solved, the infective model is difficult to be succeeded. However, the developing of the transfective model is stirring. But replicon of HCV can only transitorily replicate in minority of cells, and the produced virus is very low. The subgenomic replicon setted up in 1999 makes that people can do deep research when introduced artificial base mutation. It is a big breakthrough that a robust full-length replicon is founded lately without adaptive mutations, which could produce infectious HCV. In this article, the concise review of currently available culture system in vitro of HCV were provided.

关 键 词：丙型肝炎病毒 细胞模型 复制子 细胞培养 

分 类 号：Q813[生物学—生物工程] R373[医药卫生—病原生物学]