血清白蛋白-氯化铜-壳聚糖体系共振瑞利散射法测定壳聚糖  

Determination of Chitosan by Serum Albumin-CuⅡ -Chitosan Ternary System with Resonance Rayleigh Scattering Spectra

作　　者：杨季冬[1] 邓世星[1] 周尚[1] 

机构地区：[1]西南大学化学化工学院

出　　处：《分析化学》2007年第11期1619-1624,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No20475045);重庆市教委科研资助(NoKJ051303)项目

摘　　要：在pH 6．5的B-R缓冲溶液中，壳聚糖与血清白蛋白作用，体系的共振散射光谱较弱；当加入Cu^2＋后，使Cu^2＋与血清蛋白的浓度比为10：1时，反应形成三元配合物。三元体系的共振瑞利散射（RRS）光谱显著增强，并形成新的RRS光谱，其光谱增强程度与壳聚糖含量呈线性关系。实验考察了BSA和HSA分别与Cu（Ⅱ）和壳聚糖形成的三元体系，以及反应条件和机理，对于BSA体系，其最大RRS峰位于350nm和480nm两处，而HSA体系的最大RRS峰位于340nm处，两个体系的线性范围和检出限分别是0．01～4．0mg／L和3．7μg/L、0．01～6．0mg／L和11μg/L，其RSD均小于4．0％。显然，BSA体系有较高的灵敏度。据此可建立起RRS技术分析测定痕量壳聚糖的简便、快速和高灵敏度的新方法。同时，考察了共存物质的影响，表明方法有较好的选择性。将此法用于测定保健胶囊和自制壳聚糖粗品中壳聚糖的测定，结果满意。In pH 6.5 Britton-Robinson （B-R） buffer solution, serum album （SA） and chitosan react with each other, and the Resonance Rayleigh scattering （RRS） spectra of the system are very weak. However, after adding Cu^2＋and making the concentration ratio of Cu^2＋ and SA is 10： 1, it would react to form ternary complex, and its RRS intensities are significantly enhanced, at the same time, a new RRS spectrum would appear, and the enhanced intensity is directly proportional to the concentration of Chitosan. In this work, the reaction conditions and the mechanism of the ternary complex of BSA （ or HSA） with Cu（Ⅱ） and Chitosan were studied, the maximum RRS wavelengths are located at 350 nm and 480 nm for BSA system, and 340 nm for HSA system. The linear range and the detection limits are 0.01 -4.0 mg/L and 3.7 μg/L for BSA system, and 0.01 - 6.0 mg/L and 11 μg/L for HAS system, the RSD is less than 4.0%. It is obvious that the BSA system has higher sensibility. So, a sensitive, simple and fast method for the determination of trace amounts of chitosan by RRS technique has been developed. The influence of coexiting substances was investigated, It is showm that the method has good selectivity. The method has been applied to the determination of chitsoan in the hygienical capsule samples and self-made chitosan preform samples with satisfactory results.

关 键 词：壳聚糖 共振瑞利散射 牛血清白蛋白 人血清白蛋白 

分 类 号：O636.1[理学—高分子化学]