禽Ⅱ型副粘病毒各种禽源分离株的免疫原性  

Immunogenicity of avian paramyxovirus type 1 isolates originated from different avian species

作　　者：王林果[1] 韦平[1] 韦天超[1] 李康然[1] 黄淼章[1] 黄钰缤[1] 文艳玲[1] 杨宗维[1] 

机构地区：[1]广西大学养禽与禽病研究所,广西南宁530005

出　　处：《中国兽医学报》2007年第6期790-794,共5页Chinese Journal of Veterinary Science

基　　金：广西自然科学基金(桂科字0249006);广西玉林市科研项目(玉市科字[2002]50_5)

摘　　要：分别制备了源于鸡、鹅、鸽、鹌鹑、孔雀、画眉鸟、珍珠鸡的禽Ⅰ型副粘病毒（APMV-1）7个强毒分离毒株和商品弱毒疫苗株克隆30（C30）的灭活油乳剂苗，并用这8种灭活油乳剂苗和C30株的活疫苗分别在鸡、鹌鹑、鹅和鸽进行了免疫及交叉攻毒保护试验。免疫后（PI）分别测定试验禽血清的新城疫病毒（NDV）血凝抑制（HI）抗体的滴度，并于PI5周用强毒株进行攻毒。结果表明，鸽的HI抗体几何平均滴度（MAT）为9．00～10．0log2，鸡的为7．13～7．63log2，鹌鹑的为5．OO～5．13log2，鹅对灭活油乳剂苗的为5．63～6．38log2、而对C30株活疫苗的仅为3．38log2；除了C30株活疫苗免疫鹅提供的保护率比较低（20％）外，8种油乳剂苗都能对同源或者异源强毒株的攻毒提供比较高的保护率（66．75％～100％）。研究结果表明，经典疫苗株C30与近年来从各种不同禽类分离的致病性APMV-1野毒株之间、不同禽源分离株之间的抗原性，以及各种不同禽源分离株的之间的免疫原性差异均不大。The immunization and cross-protection test was preformed in chickens ,quails ,geese and pigeons by using the oil-emulsion vaccines （OEV） prepared from different APMV-1 isolates from chickens,geese,pigeons, quails,peacock,thrush,Guinea fowl ,and commercial attenuated vaccine strain Clone30 （C30）,respectively and live C30. Sera of the birds were collected and detected for hemoagglutinization inhibit （HI） antibody against NDV at different weeks post-immunization （PI）, and then all the birds were challenged with the virulent NDV at PI 5 weeks. The mean average titers （MAT） of HI antibody were 9. 00-10. 0 log2,7. 13-7. 63 log2,5. 00-5. 13 log2, 5.63-6.38 log2 （C30-OEV） and 3.38 log2 （live-C30） in pigeons ,chickens,geese and quails,respectively. HI titers of the group vaccinated with OEV were 5. 63-6. 38; All the eight OEV vaccines can provide good protections （66.75 %-100% ） against the challenges with homogenous and heterogeneous viruses ,except that live C30 can only provide the protection of 20% in geese. The results of the study demonstrated that there is no much difference in antigenicity and immunogenicity between the classic vaccine strain C30 and recent isolates of APMV-1 or among the isolates.

关 键 词：禽Ⅰ型副粘病毒 免疫原性 抗原性 血凝抑制抗体滴度 免疫交叉保护试验 

分 类 号：S852.65[农业科学—基础兽医学]