口蹄疫重组鸡痘病毒活载体疫苗在豚鼠、猪体内的毒性、分布及抗体消长规律  

Distribution,virulence and antibody variation of recombinant fowlpox virus vUTAL3CP in cavia cobaya and swine

作　　者：金明兰[1] 金宁一[1] 鲁会军[1] 马鸣潇[2] 郑敏[2] 霍小伟[2] 刘慧娟[2] 计越[2] 李旭[2] 

机构地区：[1]军事医学科学院军事兽医研究所,吉林长春130062 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2007年第6期803-806,共4页Chinese Journal of Veterinary Science

基　　金：吉林省重大科技资助项目(2004-0202-1)

摘　　要：利用临床观察、病理解剖、PCR、RT-PCR、ELISA、中和抗体检测等方法,对口蹄疫(FMD)重组鸡痘病毒(FPV)在豚鼠、仔猪体内的毒性、分布以及抗体消长规律进行研究。结果表明,FMD重组鸡痘病毒免疫的动物在整个试验期间,未表现出明显的临床症状和不良反应;病理组织切片检测无明显的组织学变化;PCR、RT-PCR检测证明,豚鼠、猪免疫FMD重组鸡痘病毒后,在心脏、肝脏、脾脏、肺脏、肾脏、肌肉、脑、肠系膜淋巴结内检测到FPVDNA和FMDV DNA,且在大部分组织能存在3 d左右;FMD重组鸡痘病毒均可诱导免疫动物产生较高水平的抗FMDV特异性抗体和中和抗体,验证了所构建重组FPV的生物安全性及良好的免疫原性,为其他哺乳动物实验提供了必要的基础数据。The clinical, histological, viral culture, PCR, neutralizing antibody were used to assess the bio-safety and immunogenicity of the recombinant fowlpox virus. During the experiment, no detectable pathological changes were found in the organs of inoculated cavia cobaya and swine. Using PCR, the viral nucleic acids was detected at 3 days post inoculation （DPI）, viral nucleic acids were found in all organs; none viral nucleic acids were detected from any organs at 10 DPI. In addition, well immunogenicity had been ensured by its ability to induce humoral responses.

关 键 词：口蹄疫重组鸡痘病毒 毒性 分布抗体消长规律 豚鼠 猪 

分 类 号：S852.65[农业科学—基础兽医学]