用单胚mRNA差显方法克隆羊早期胚胎发育阻滞形成的相关基因  

Cloning of goat embryo development block related genes using single preimplantation embryo mRNA differential display

作　　者：李拥军[1] 敖红[2] 孙桂金[2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院畜牧研究所,北京100094

出　　处：《中国兽医学报》2007年第6期959-962,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30270162);北京市自然科学基金资助项目(6032019)

摘　　要：为探讨山羊体外培养胚胎发育阻滞的发生机理，用单胚mRNA差异显示技术，对来源于体内发育和体外培养的山羊早期8～16细胞期胚胎的基因表达进行了研究，获得体内发育胚胎特异表达的一些片段，并对其中1个片段进行了分析。结果表明，该片段与犬信号肽酶复合体25亚基（SPC25）基因具有92％的同源性。SPC蛋白可通过切除相关前体蛋白的信号肽，成为成熟的分泌蛋白而影响和作用于早期胚胎发育过程。该基因可望对克服早期胚胎发育阻滞有重要作用。In order to study the mechanism about the development block of goat preimplantation embryos, the goat 8-16C stage embryos obtained from in vivo and in vitro was studied using the way of single preimplantation embryo mRNA differential display. Some specific fragments expressed in in vivo embryos was found and one of them was selected for further analysis. The result suggested that this fragment displayed high homology （92%） to dog signal peptidase complex 25 000 subunit （SPC25） gene. Matured secretory proteins obtained when the signal peptides of its pre-protesome were cut out by SPC, and these secretory proteins revealed the functions on the embryo development. Thereby it is believed that the lack of the gene expression of SPC25 may account for the development block of in vitro produced goat implantation embryos.

关 键 词：单胚差显技术 山羊 胚胎 发育阻滞 基因 

分 类 号：S814.8[农业科学—畜牧学]