玉米C型细胞质雄性不育特异片段的分离及验证  

Separation and verification of a specific AFLP fragment from the C-type cytoplasmic male sterility in maize

作　　者：许珂[1] 曹墨菊[2] 朱英国[2] 潘光堂[1] 荣廷昭[1] 

机构地区：[1]四川农业大学玉米研究所／四川农业大学教育部作物基因资源与遗传改良重点实验室,雅安625014 [2]武汉大学植物发育生物学教育部重点实验室,武汉430072

出　　处：《高技术通讯》2007年第11期1180-1183,共4页Chinese High Technology Letters

基　　金：教育部长江学者和创新团队发展计划（IRT0453）和四川省教育厅（2003A019）资助项目.

摘　　要：对一套玉米同核异质不育系及保持系（包括N48-2、T48-2、C48-2、S48-2）的线粒体DNA进行了扩增片断长度多态性（AFLP）分析比较。从选择性扩增产物中筛选到了一条C48-2所特有的差异条带C48-223。对C48-223进行克隆、测序分析表明,它是玉米C型细胞质雄性不育线粒体atp6基因编码序列的一部分。Southern、Northern杂交分析证实了C48-223在C48-2线粒体上的特异性。这说明该差异片段有可能同C型细胞质雄性不育性状的表现有关。The analyses of mitochondrial DNA extracted from one set of isonuclear alloplasm materials including N48-2, T48- 2, C48-2 and S48-2 in maize and the comparison between them were carried out with AFLP technique. C48-223, one differential DNA segment of C48-2, was found when the mtDNA fragments were amplified with selective primers. The band was isolated, purified and sequenced, and the results showed that C48-223 was a part of atp6-C, which is a chimeric gene uniquely associated with the C male-sterile cytoplasm of maize. The results of Southern blot indicated that C48-223 was a real band. Via Northern blot, the signals can only be detected at the tetrad stage and uninucleate stage of another RNA extracted from C48-2. All these indicate that the fragment probably has strong relationship with the C male-sterile cytoplasm of maize.

关 键 词：玉米 细胞质雄性不育 线粒体DNA 扩增片断长度多态性(AFLP) 

分 类 号：S565.4[农业科学—作物学]