羧甲基-β-环糊精手性流动相添加剂法拆分帕罗西汀及其中间体对映体  

Separation of Paroxetine and Its Intermediate Enantiomers by High Performance Liquid Chromatography Using Carboxymethyl-β-Cyclodextrin as Chiral Mobile Phase Additive

作　　者：卢铁刚[1] 杨茂俊[1] 

机构地区：[1]四川抗菌素工业研究所,四川成都610051

出　　处：《色谱》2007年第6期830-833,共4页Chinese Journal of Chromatography

摘　　要：建立了帕罗西汀及其中间体的高效液相色谱手性拆分分析方法。选用C18柱(4.6mm×250mm,5μm),流动相为0.1%磷酸-甲醇(体积比为65∶35,含0.38g/L羧甲基-β-环糊精,以三乙胺调pH7.2),柱温25℃,检测波长210nm。结果表明,帕罗西汀及其中间体HFP的对映异构体在30m in内同时得到了基线分离,该法与手性固定相法相比具有分离效果更好的优势。A high performance liquid chromatographic method to separate the respective enantiomers of paroxetine and its intermediate was developed using chiral mobile phase additive. Separation was performed on a Diamond C18 column （4.6 mm×250 mm, 5μm）. The. mobile phase was 0. 1% phosphate acid-methanol （55： 35, v/v） containing 0.38 g/L carboxymethyl-β- cyclodextrin and the pH was adjusted to 7.2 by triethylamine. The detection wavelength was set at 210 nm and the temperature was 25 ℃. With this method, paroxetine＇ s trans/cis isomers and their enantiomers as well as intermediate HFP＇ s trans/cis isomers and their enantiomers were separated simultaneously. The method is simple, rapid with high resolutions.

关 键 词：高效液相色谱 手性流动相添加剂 羧甲基-β-环糊精 对映体分离 帕罗西汀 

分 类 号：O658[理学—分析化学]