食管鳞癌患者外周血T淋巴细胞亚群的变化  

The alterations of T lymphocyte subsets in peripheral blood of the patients with esophageal squamous cell carcinoma

作　　者：李进东[1] 王文光[1] 许金良[1] 赵婷茹[2] 

机构地区：[1]河南省肿瘤医院胸外科,郑州450008 [2]河南省肿瘤医院病理科

出　　处：《中国医师进修杂志（外科版）》2007年第11期35-37,40,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的研究食管鳞癌患者外周血T淋巴细胞亚群的变化特点。方法采用流式细胞技术，对108例食管鳞癌患者（食管鳞癌组）术前检测其外周血T淋巴细胞亚群水平，并与50例健康献血者（对照组）对比，分析食管鳞癌患者T淋巴细胞亚群的变化特点。结果（1）与对照组比较，食管鳞癌组患者外周血CD3^＋ 、NK细胞水平差异无统计学意义（P=0．890；P=0．320）；CD4^＋和CD8^＋水平显著增高（P=0．001；P〈0．001），由于CD8^＋增高的程度大于CDZ增高的程度，使得CDgCDs~较对照组降低（P〈0．001）。（2）早期食管鳞癌患者外周血CD4^＋/CD8^＋低于对照组，但差异无统计学意义（P=0．112）；中晚期食管鳞癌患者CDgCDs~进一步降低，与对照组比较差异有统计学意义（P〈0．001），但与早期食管鳞癌患者相比，差异无统计学意义（P=0．218）。（3）有淋巴结转移食管鳞癌患者CD4^＋和CD4^＋/CD8^＋均低于无淋巴结转移患者（P均〈0．001），CD8^＋和NK细胞均高于无淋巴结转移患者，差异均有统计学意义（P=0．002；P=0．005）。结论食管鳞癌患者存在免疫功能低下，随着肿瘤的进展免疫功能进一步受到削弱；监测CD4^＋/CD8^＋的变化可作为食管鳞癌病情进展的标志之一。Objective To investigate the alteration of T lymphocyte subsets in patients with esophageal squamous cell carcinoma. Methods T lymphocyte subsets were detected in peripheral blood by flow cytometry. Blood samples were obtained preoperatively in 108 patients with esophageal squamous cell carcinoma（patients group）. As control group ,blood samples were also obtained from 50 healthy blood donors. Results There was no statistical difference in CD3^＋ and NK cells （nature killer cells） value between patients group and control group（P = 0.890, P = 0,320 ）. CD4^＋ and CD8^＋ value in patients group were significantly higher than those in control group （P= 0,001 ,P〈 0.001 ）. Due to CD8^＋ increased to a greater extent than CD4^＋ did, CD4^＋/CD8^＋ ratio was significantly lower than that in control group （P 〈 0.001 ）. CD4^＋/CD8^＋ ratio decreased in patients with early stage carcinoma, but compared with that in control group, there was no significant difference （P = 0.112）. CD4^＋/CD8^＋ ratio further decreased in patients with advanced carcinoma,compared with that in control group,there was significant difference （P 〈 0.001 ）,while compared with that in patients with early stage carcinoma, there was no significant difference（P = 0.218 ）, CD4^＋ value and CD4^＋/CD8^＋ ratio showed significant decrease in patients with lymph nodes metastasis than that in patients without lymph node metastasis （P 〈 0.001 ）. While CD8^＋ and NK cells were both significantly higher in patients with lymph node metastasis than those in patients without lymph node metastasis （P = 0.002,P = 0.005）. Conclusions The host im- munity is impaired in patients with esophageal squamous carcinoma. With the progression of esophageal squamous carcinoma,the immunological function was further suppressed. Monitoring the change of CD4^＋/CD8^＋ ratio might served as one of the markers of the progression of esophageal squamous carcinoma.

关 键 词：食管 鳞状细胞癌 外周血 T淋巴细胞亚群 

分 类 号：R725.622.5[医药卫生—儿科]