脂蛋白脂酶基因Ser447Ter多态性与2型糖尿病的相关性  

Association between lipoprotein lipase Ser447Ter polymorphism and type 2 diabetes mellitus

作　　者：黄融[1] 刘伟[1] 胡耀敏[1] 张小英[1] 陈雅文[1] 

机构地区：[1]上海交通大学医学院仁济医院内分泌代谢病科,上海200127

出　　处：《上海交通大学学报（医学版）》2007年第11期1341-1345,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30670988)~~

摘　　要：目的探讨上海地区汉族人群脂蛋白脂酶(LPL)基因Ser447Ter多态性与2型糖尿病患者糖、脂代谢的相关性。方法随机选取上海地区无血缘关系汉族人437例。其中2型糖尿病患者240例(糖尿病组),糖耐量正常者197例(对照组)。糖尿病组再根据空腹甘油三酯(TG)水平分为为正常TG组(T2DM-NTG亚组,TG<1.70 mmol/L)和高TG组(T2DM-HTG亚组,TG≥1.70 mmol/L)。比较各组人群的糖、脂代谢指标;采用PCR-RFLP法检测各组LPL基因Ser447Ter多态性,并分析其与2型糖尿病患者糖、脂代谢指标的关系。结果糖尿病组LPL基因Ser447Ter多态性位点等位基因、基因型频率显著低于对照组(P<0.01);T2DM-HTG亚组该位点等位基因、基因型频率显著低于T2DM-NTG亚组(P<0.05);糖尿病组Ter等位基因携带者与非携带者相比,空腹TG、总胆固醇、血糖、胰岛素水平及胰岛素抵抗指数(HOMA-IR)均显著降低(P<0.05)。结论LPL基因Ser447Ter多态性在2型糖尿病发生过程中,可能是一保护性因素,表现为TG降低和胰岛素抵抗改善。Objective To explore the relationship between Ser447Ter polymorphism in the lipoprotein lipase （LPL） gene and glucose and lipid metabolism in type 2 diabetic patients from a population of Han Chinese in Shanghai. Methods Four hundred and thirty-seven Han Chinese with no blood relationship were randomly selected in Shanghai, among whom 240 were type 2 diabetic patients （diabetics group） and 197 were normal glucose tolerance subjects （control group）. The patients in the diabetes group were further divided into 2 subgroups by the level of fasting triglyceride （TG） with the borderline of 1.70mmol/L, the T2DM-NTG subgroup （TG 〈 1.70 mmol/L） and the T2DM-HTG subgroup （TG≥ 1.70 mmol/L）. The glucose and lipid metabolism parameters were compared among the groups. The LPL Ser447Ter polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method, and its association with glucose and lipid metabolism in type 2 diabetic patients was investigated. Results The allele and genotype frequencies of Ser447Ter polymorphism of LPL gene in the diabetes group were significantly lower than those in the control group （ P 〈 0.01 ）. And those in the T2 DM-HTG subgroup were significantly lower than those in the T2 DM-NTG subgroup （ P 〈 0.05）. For the patients in the diabetes group, those carrying allele Ter had lower levels of fasting TG, total cholesterol, plasma glucose, insulin and HOMA-IR（P 〈0.05）. Conclusion The Ser447Ter polymorphism of LPL gene may have a protective role against the development of type 2 diabetes, which is embodied by favorable effects on TG and insulin resistance.

关 键 词：脂蛋白脂酶 单核苷酸多态性 2型糖尿病 高甘油三酯血症 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌] Q754[医药卫生—内科学]