人工神经支架材料聚（羟基乙酸-L-赖氨酸-乳酸）／NGF／β-TCP复合膜的制备及性能研究  

Preparation and characterization of nerve graft material poly[LA-（GIc-Lys）]/NGF/β-TCP

作　　者：李娟[1] 严琼姣[1] 徐盈 李世普[1] 

机构地区：[1]武汉理工大学生物材料与工程研究中心,湖北武汉430070

出　　处：《功能材料》2007年第A05期1851-1853,共3页Journal of Functional Materials

基　　金：国家重点基础研究发展计划（973计划）资助项目（20005CB623905）

摘　　要：通过对聚乳酸进行物理和化学改性，制备了一种用于神经修复的新型组织工程支架材料——霖（羟基乙酸-L-赖氨酸-乳酸）／NGF／β-TCP。采用FT-IR，^1H-NMR对中间产物和目标聚合物进行了表征。材料接触角的测试显示该材料亲水性较聚乳酸有明显改善；采用MTT实验检测细胞活性，其OD值明显高于对照组聚乳酸：通过免疫荧光观察细胞在材料表面的黏附与生长情况，结果显示嗅鞘细胞在该材料上生长形态及铺展状况良好。Poly[LA-（Glc-Lys）]/NGF/β-TCP as a novel nerve graft material was prepared by modified poly lactic acid with Physical and chemical methods. And the in-process product and target copolymer were characterized by FF-IR and ^1H-NMR. Water Contact angle test indicated the copolymer had much better hydrophilicity than PLA. Olfactory ensheathing cells were co-cultured with poly[LA-（Glc-Lys）]/NGF/β-TCP film and PDLLA film to evaluate their cell-compatibility. The MTT test shows that the poly[LA-（Glc-Lys）]/ NGF/ β-TCP film is superior in cell activity to PDLLA film, while the immunofluorescence shows Poly[LA-（Glc-Lys）]/β-TCP composite has good cell-compatibility and can promote the growth of OECs.

关 键 词：神经修复 聚(羟基乙酸-L-赖氨酸-乳酸)/NGF/β-TCP 细胞相容性 

分 类 号：R313.08[医药卫生—基础医学]