四川省致病性钩端螺旋体脉冲场电泳分型分析  

Molecular Typig of Leptospira Interrogans From Sichuan Province by Pulsed-field Gel Electrophoresis

作　　者：郭宗琪[1] 黄自英[1] 陈娜[1] 罗隆泽[1] 徐竹清[1] 

机构地区：[1]四川省疾病预防控制中心,四川成都610041

出　　处：《预防医学情报杂志》2007年第6期644-646,共3页Journal of Preventive Medicine Information

基　　金：四川省卫生厅病原微生物分子生物学重点实验室建设项目资金

摘　　要：目的建立和应用脉冲场电泳(PFGE)分型技术,研究四川省致病性钩端螺旋体的分子流行病学。方法应用DNA限制性内切酶NotⅠ,对钩体野生株染色体DNA酶切后,用PFGE进行分子分型。利用BiOnurericsV3.0建立数据库和相似性聚类。结果来自四川省不同地区的140株钩体可分为64个PFGE型,菌株相似值在67%～100%之间。同一地区患者和宿主动物分离株有相同的图谱。结论PFGE分型分析可用于钩端螺旋体遗传学分类研究,在分子流行病学研究中有较大意义。Objective To understand the genetic typing naps of Leptospira interrogns in SiChuan province by pulsed-field gel electrophoresis（PFGE）. Methods The chrumosoml DNAs were digested with restriction endo- nuclease Not I and the fragments were separated by PFGE and clustering of patterns was analyzed with Bion- umerisc safiware. Results 64 different molecular types were among 140 strains of Leptospira interrogns. The similarity of used strains was in the range from 67% to 100%. Nomologoustrains from people and animals were discovered in the same areas. Conclusion PFGE patterns could be used to classify and identify in molecular epidemiological research of Leptospirosis.

关 键 词：钩端螺旋体 脉冲场电泳 基因分型 

分 类 号：R377.5[医药卫生—病原生物学]