血清对氧磷酶活性测定条件研究  

Factors that Influence Detection of Serum Paraoxonase Activity

作　　者：李园园[1] 李萍[1] 

机构地区：[1]四川大学华西医院实验医学科,四川成都610041

出　　处：《预防医学情报杂志》2007年第6期677-679,共3页Journal of Preventive Medicine Information

摘　　要：目的探讨不同底物浓度、Ca2+浓度、缓冲液浓度及pH情况下酶的活性,摸索酶活性测定的最适条件。方法通过改变底物、Ca2+、缓冲液浓度及pH值,在半自动生化分析仪上测定对氧磷酶活性,确定最适反应条件。结果酶促反应产物对硝基酚的吸收峰为405nm,摩尔消光系数为17143,酶促反应的最适条件:0.4mol/L甘氨酸、2mmol/LCaCl2、2mmol/L对氧磷、pH10。结论可以用此条件建立血清对氧磷酶的测定,但是试剂必须临用前配制。Objective To establish a most suitable condition for detecting serum paraoxonasel （ PON1 ） activity. Methods The PON1 activity was determined by kinetic method in semiautomatic biochemical analyzer under different concentrations of substrate, Ca^2＋ and buffer, PH. Results The suitable condition for detection was 0. 4 mol/L glycine, 2 mmol/LCaCk2, PH 10 and 2mmol/L paraoxon. Conclusion The method can be used to detect serum paraoxonase activity, but reagent must be prepared just before using.

关 键 词：对氧磷酶 活性 对氧磷 

分 类 号：R446.1[医药卫生—诊断学]