巢蛋白和阶段特异性胚胎抗原-1在O-2A谱系细胞中的表达  

The Expression of Nestin and Stage Specific Embryonic Antigens-1 in O-2A Lineage Cells

作　　者：李春鹏[1] 孙文阁[1] 夏春林[1] 

机构地区：[1]苏州大学细胞神经生物学研究室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2007年第2期176-177,188,F0002,共4页Suzhou University Journal of Medical Science

基　　金：江苏省自然科学基金资助课题(No.BK1999084);江苏省高校自然科学研究计划(No.05KJB310121);江苏省分子神经生物学重点

摘　　要：目的观察O-2A(oligodentrocyte-type-2 astrocytes)谱系细胞是否是表达神经干细胞的标志物。方法取新生大鼠脑皮质体外培养纯化的O-2A祖细胞、少突胶质细胞和2型星形胶质细胞,应用激光共聚焦双重免疫荧光标记技术检测巢蛋白(nestin)和阶段特异性胚胎抗原-1(SSEA-1)的表达。结果nestin在O-2A祖细胞和2型星形胶质细胞中表达,在少突胶质细胞中不表达;SSEA-1仅在2型星形胶质细胞中表达。结论nestin和SSEA-1在O-2A谱系细胞中的表达存在差异,可能具有神经干细胞的特征。Objective To investigate whether oligodendrocytes（OL） and type-2 astrocytes（T2A） which derived from oligodendrocyte-type-2 astrocytes（O-2A） progenitor cells express the characteristic molecules of neural stem cells. Methods Purified cultures of cortical O-2A progenitor cells were prepared from neonatal rats. The differentiation into oligodendrocytes and T2A was achieved by using N2 medium and 10%FCS respectively. The expression of nestin and stage specific embryonic antigens-1 （SSEA-1） was detected by immunocytochemical labeling. Results O-2A progenitor cells and T2A were immunoreactive for nestin, while oligodendrocytes showed no nestin staining. Expression of SSEA-1 was only observed in T2A. Conclusion Oligodendrocytes and T2A show differential expression patterns of nestin and SSEA-1. The results indicate that T2A may share some identities of the neural stem cells.

关 键 词：O-2A谱系细胞 巢蛋白 阶段特异性胚胎抗原-1 免疫细胞化学 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]