兔腰椎间盘退变模型的建立及影像学分析  

Animal Model Development and Imaging Analysis of the Degenerative Disc in Rabbit

作　　者：吴健[1] 唐天驷[1] 王根林[1] 赖震[1] 殷浩[1] 祝晓[1] 

机构地区：[1]苏州大学附属第一医院骨科,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2007年第4期552-554,共3页Suzhou University Journal of Medical Science

摘　　要：目的探讨针刺抽吸法建立椎间盘退变动物模型的可行性。方法新西兰大白兔18只,用持针器夹持21 G皮肤穿刺针从椎间隙正前方刺入L3-4、L4-5、L5-6椎间盘的纤维环,深度控制在5 mm,拔出针芯,抽吸出部分髓核组织。术前及术后4、8、12周分别对造模后椎间盘及对照椎间盘(L2-3)行MRI及CR检查。结果术后第4周到第12周,造模后的椎间盘MRI的T2W1信号呈现持续减弱趋势;椎间盘高度指数持续下降,差异有高度统计学意义(P<0.01)。结论针刺抽吸法可诱导兔椎间盘的缓慢退变,从而为研究椎间盘退行性变的机制及治疗提供有效的动物模型。Objective To establish a slowly progressive reproducible rabbit model of intervertebral disc degeneration induced by puncturing the annulus fibrosus with needles of defined gauges and aspi- rating some nucleus pulposus tissue from the intervertebral discs（IVDs）. Methods The L3-4, L4-5 and L5-6 lumbar intervertebral discs of 18 New Zealand white rabbits were stabbed by 21-gauge hypodemic needle into a depth of 5 mm in the antero annulus fibrosus, the needle valve was pulled out, and some nucleus pulposus tissue aspirated out. Magnetic resonance imaging scans （MRI） and computed radiography （CR） analysis of the stabbed discs and intact L2-3 disc were performed preoperatively and at the 4th, 8th,12th week respectively after surgery. Results In the MRI the stabbed discs a progressive decrease of sigal intensity was exhibited in T2-weighted images which start at the 4th week after stabing and last for 12 weeks. CR analyses indicated progressive decrease of the disc height index （P 〈 0.01 ）. Conculation The stabbing and asprirating approach results in a slowly progressive intertebral disc degeneration in rabbit model. This model is available for studying the status of intervertebral disc dege-neration and treatment.

关 键 词：腰椎间盘退变 模型 动物 针刺抽吸法 兔 

分 类 号：R681.53[医药卫生—骨科学]