火焰原子吸收光谱法研究五种螫合剂对染镉小鼠睾丸中镉钙铁锌浓度的影响  

FAAS Investigation of Therapeutic Effect of Chelating Agents on Cd,Ca,Fe,Zn in Testis of Acutely Cd-Infected Mice

作　　者：陈敏[1] 谢吉民[1] 闫永胜[1] 

机构地区：[1]江苏大学化学化工学院,镇江212013

出　　处：《理化检验（化学分册）》2007年第9期709-712,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：江苏省青蓝工程新世纪学科带头人基金及江苏省教育厅自然科学基金(00KJB330003)资助。

摘　　要：采用火焰原子吸收光谱法分别对染镉小鼠和N-对羟甲苯甲基-D-葡糖二硫代氨基甲酸钠(HBGD)、N-苯甲基-D-葡糖二硫代氨基甲酸钠(BGD)、二乙基二硫代氨基甲酸钠(DDTC)、二巯基丙醇(BAL)、乙二胺四乙酸(EDTA)等五种螯合剂治疗组小鼠睾丸中镉、钙、铁、锌等金属浓度进行了测定和研究。结果表明:镉染毒(Cd 2.5 mg·kg^(-1),腹腔注射)后,小鼠睾丸中镉、钙、铁、锌等金属浓度显著高于对照组(p<0.05),染镉30 min后用各螯合剂治疗(0.400 mmol·kg^(-1),腹腔注射),24 h后除EDTA外,其余螯合剂能明显降低睾丸中镉的浓度并抑制钙、铁、锌浓度的增加(p<0.05);染镉24 h后注射各螯合剂,治疗24 h后,HBGD、BGD和DDTC显著降低了小鼠睾丸中各金属浓度(p<0.05);提示HBGD和BGD有望成为染镉中毒的理想解毒剂。For purpose of developing new antidotes for Cd-infection,a FAAS method for the determination of Cd,Ca,Fe and Zn in testis of Cd-infected mice was established.The mice were infected by injection of CdCl_2 solution into the abdominal cavity（2.5 mg·kg^（-1））and the mice of control group were injected with plysiological saline.Determination results showed that in testis of the CA-infected mice,concentration of Cd,Ca,Fe,Zn was significantly increased,compared to the controlled group（p〈0.05）.Several chelating agents were selected as the antidote candidates for the therapeutic effect investigation.They are N-p-hydroxy-methyl-benzyl-D-glcamine di- thioearbamate（HBGD）,N-benzyl-D-glucamine di-thiocarbamate（BGD）,diethyl-dithiocarbamate（DDTC）,2,3- dimercaptopropanol（BAL）and ethylene diamine tetra acetic acid（EDTA）.The chelating agent was also injected into the abdominal cavity（0.400 mmol·kg^（-1））as the therapy.Experimental findings verified that after 30 min of thesapeutic injection,the chelating agents,with exception of EDTA,significantly decrease the concentrations of CA,Ca,Fe,Zn（p〈0.05）,and after 24 h of therapeutic injection,HBGD,BGD and DDTC display a notable effect of the decrease in Cd,Ca Fe,Zn concentrations.Considering the self toxicity of the chelating agents,it is suggested that HBGD and BGD are the better antidotes for Cd-infection.

关 键 词：火焰原子吸收光谱法 螯合剂 解毒剂 睾丸 镉 钙 铁 锌 

分 类 号：O657.31[理学—分析化学] O593.9[理学—化学]