通心络抑制白细胞介素1β介导的小型猪冠状动脉早期炎症反应及内膜增殖  

Tongxinluo Inhibiting Coronary Artery Early Inflammation Response and Intima Proliferation Induced by Interleukin-1β in Small Swine

作　　者：关启刚[1] 曾定尹[1] 孙喜琢[2] 周旭晨[1] 程颖[1] 苗志林[1] 何学志[2] 韩凤桐[2] 张利[2] 

机构地区：[1]中国医科大学附属第一医院心内科,辽宁省沈阳市110001 [2]大连市中心医院心内科,辽宁省大连市116033

出　　处：《中国动脉硬化杂志》2007年第8期575-579,共5页Chinese Journal of Arteriosclerosis

基　　金：国家重点基础研究发展计划(973计划)资助项目(2005CB523310)

摘　　要：目的探讨通心络对白细胞介素1β介导的小型猪冠状动脉早期炎症反应及内膜增殖的抑制作用及可能机制。方法24头小型猪随机均分为假手术组、模型组和通心络组。假手术组和模型组喂养普通饲料,通心络组喂养普通饲料＋通心络[1 g/（kg.d）]。2周后麻醉行左侧开胸手术,选择左前降支及回旋支近端管腔外径近似相同的两处节段进行仔细分离,假手术组在血管外膜包裹吸附生理盐水的纸巾,模型组和通心络组包裹吸附白细胞介素1β（2.5μg）的纸巾,术后继续以上喂养方法。2周后采用冠状动脉造影观察各组管腔狭窄情况。造影结束后处死动物,截取包裹血管段,进行病理学检查,并采用逆转录聚合酶链反应,测定各组手术处理血管段单核细胞趋化蛋白1、P选择素、E选择素、细胞间粘附分子1和血管细胞粘附分子1 mRNA的表达。结果冠状动脉造影显示,模型组冠状动脉管腔20%~30%狭窄,病理学检查可见模型组内膜增殖、炎性细胞浸润,平滑肌细胞内膜下迁移。通心络组管腔狭窄程度、内膜增殖及炎性细胞浸润现象较模型组明显减轻。逆转录聚合酶链反应示通心络组单核细胞趋化蛋白1、P选择素、E选择素、细胞间粘附分子1和血管细胞粘附分子1 mRNA表达强度低于模型组（P〈0.05）。结论通心络对白细胞介素1β诱导的冠状动脉炎症反应及内膜增殖具有明显的抑制作用。通过抑制细胞因子和粘附因子的表达可能是其主要作用途径。Aim To observe file inhibition effect of Tongxinluo on inflammation and intima proliferation response induced by interleukin-1β （IL-1β） in small swine, and to investigate its action mechanism. Methods Clfinese mini-swine were randomly divided into three groups, control group, model group and Tongxinluo group. The control group and model group were fed with special diet; the Tongxinluo group were fed with special diet and Tongxinluo [ 1 g/（kg·d） ]. After 2 weeks＇ feeding, chest operation was done by isolation of left-anterior descending banch and circumflex branch proximate, Coronary artery membranna extema in swine was packed by paper towel absorbing saline agarose particle suspension in control group. But in model group and Tongxinluo group, coronary artery membranna externa was packed by paper towel absorbing IL-1β （2.5 μg）agarose particie suspension. After 2 weeks, lumens narrow was observed by coronary artefiogramphy. The vascular section in the treated site was taken out to he exanfined by pathology and the monocyte dhemoattractant protein-1 （MCP-1）, P-selection, E-selection, inter-cell adhesion molecale-1 （ICAM-1） and vascular cell adhesion molecale-1（VCAM-1） mRNA expression was measured by reverse transcription polymerase chain reaction analysis （RT-PCR）, Results The coronary arteriogramphy showed that atheroscierotic changes and luminal stricture in local blood vessel in Tongxinluo group was dscreased compared with control group. The pathological exarnination showed that the degree of luminal stricture, the macrophage accumulation and endometfial hyperplasia in Tongxinluo group was obviously lighter than teat of model group, The expression of mRNA of MCP-1, P-selection, E-selection, ICAM-1 and VCAM-1 in Tongxinluo group were decreased, Conclusions Tongxinluo could inhibit coronary, artery, early inflammation response and intima proliferation induced by interleukin-1β by inhibiting the mRNA expression of MCP-1, P-selection, E-selection, ICAM-I and VCAM-I.

关 键 词：内科学 动脉粥样硬化 通心络 白细胞介素1 单核细胞趋化蛋白1 炎症反应 小型猪 

分 类 号：R5[医药卫生—内科学]