糖尿病和球囊损伤对兔食饵性动脉粥样硬化形成的影响  

Effects of Diabetes and Balloon Injury on Diet Induced Rabbit Atherosclerosis

作　　者：高玲[1] 陈琴[1] 康丽娜[1] 王涟[1] 宋杰[1] 江时森[2] 徐标[1] 

机构地区：[1]南京大学医学院附属鼓楼医院心内科 [2]中国人民解放军南京军区总医院心内科,江苏省南京市210008

出　　处：《中国动脉硬化杂志》2007年第8期591-594,共4页Chinese Journal of Arteriosclerosis

基　　金：江苏省自然科学基金(BK2004083)资助

摘　　要：目的观察糖尿病和球囊损伤血管内皮对兔食饵性动脉粥样硬化形成的影响。方法球囊法损伤腹主动脉内皮加高脂饲料喂养建立兔动脉粥样硬化模型,其中1/2动物损伤内皮前1周耳缘静脉推注四氧嘧啶诱发糖尿病建立糖尿病动脉粥样硬化模型。手术后10周处死动物,通过H&E染色和免疫组织化学观察胸主动脉和腹主动脉内膜增生情况及其内巨噬细胞数量、平滑肌细胞密度,比较无内皮损伤的胸主动脉和损伤内皮的腹主动脉粥样硬化病变的不同,以及糖尿病对动脉粥样硬化病变的影响。结果糖尿病和非糖尿病兔胸、腹主动脉均形成了动脉粥样硬化病灶,但胸主动脉内膜与中膜比值(分别为0.9±0.18和0.7±0.2)显著低于腹主动脉(分别为6.9±2.0和6.9±2.1);两组兔腹主动脉内膜增生程度虽相似,但糖尿病兔粥样硬化病变内巨噬细胞浸润明显重于非糖尿病兔(阳性染色面积占内膜面积百分比分别为29.6%±5.5%和39.7%±8.7%),而平滑肌细胞密度低于非糖尿病兔(阳性染色面积占内膜面积百分比分别为38.7%±5.8%和35.2%±4.9%),这在内膜内1/2部分尤其显著(巨噬细胞阳性染色面积占内1/2内膜面积百分比分别为18.8%±4.8%和33.9%±6.2%;平滑肌细胞分别为32.8%±5.8%和25.4%±3.8%)。结论球囊损伤内皮加速了糖尿病和非糖尿病兔食饵性动脉粥样硬化病变进展;糖尿病增强了动脉粥样硬化病灶内炎症反应。Aim To study the effects of diabetes and balloon injury on diet induced arterial atherosclerosis in rabbit. Methods Set up rabbit atherosclerotic model by high cholesterol diet and injury of abdomen aorta＇s endothelium with balloon; half of the animals were injected alloxan one week before endothelium injury to make diabetic atherosclerotic model, atherosclerotic rabbits were injected physiological saline instead. Ten weeks after the surgery rabbits were sacrificed by euthanasia, transverse sections of the aorta were taken for H＆E stain and immunohistochemistry to analyze intima incrassaition, quantities of macrophages and smooth muscle cells. Results Thoracic and abdomen aorta of both diabetic and non-diabetic rabbits developed atherosclerosis, and the ratio of intima to media thickness in thoracic aorta （0.9 ± 0.18 and 0.7 ± 0.2 repectively） was much lower than in abdomen aorta （ 6.9 ±2.0 and 6.9 ± 2.1 repectively）. Though intima thickness of the abdomen aorta was similar between the two groups, diabetic atherosclerotic rabbits had more macrophages （ percentage of positive stain area in the intima was 39.7 ± 8.7 ） and fewer smooth muscle cells （ percentage of positive stain area in the intima was 29.6 ± 5.5 ） in its intima than non-diabetic atherosclerotic rabbits （ percentage of positive stain area of macrophages were 38.7 ± 5.8 and that of smooth muscle cells were 35.2 ± 4.9）, especially at luminal 1/2 of intima （percentage of positive stain area of macrophages were 18.8±4.8 and 33.9± 6.2 respectively and that of smooth muscle cells were 32.8± 5.8, 25.4 ± 3.8 respectively ）. Condusion Balloon injury of the endothelium accelerates atheresclerosis induced by diet in diabetic and non-diabetic rabbits; diabetes promotes inflammation of the atherosclerosis.

关 键 词：内科学 血管内皮 球囊损伤 糖尿病 动脉粥样硬化形成 实验动物 家兔 

分 类 号：R5[医药卫生—内科学]