微生物絮凝剂的制备及其絮凝活性  

Production and flocculation activities of microbial flocculants

作　　者：吴焕利[1] 冯贵颖[2] 赵淑艳[2] 张雪峰[1] 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨凌712100 [2]西北农林科技大学理学院,陕西杨凌712100

出　　处：《环境污染与防治》2007年第11期858-861,共4页Environmental Pollution & Control

基　　金：2004年第一批中央环境保护专项基金资助项目

摘　　要：从土壤和活性污泥中筛选获得性能稳定、对高岭土悬浮液的絮凝率达75%以上的菌株20株,其中编号为Ⅰ-18的菌株培养液对高岭土悬浮液的絮凝率达85%以上,经鉴定为球形芽孢杆菌Bacillus sphaericus。实验表明,该菌株以质量分数为2%的蔗糖为碳源,以质量分数均为0.15%的蛋白胨与尿素组合为氮源;生长与分泌絮凝剂的合适温度为30℃,适宜的pH为7.0,最佳培养时间为80h。利用该微生物絮凝剂处理水性油墨废水效果明显,15min浊度去除率达98.7%。20 bacterial strains that can produce microbial flocculants were isolated from activated sludge and soil by means of the regular bacteria isolation and purification procedure. And a particular strain, named Ⅰ-18, which can produce microbial flocculant with a flocculating efficiency of 85 % against kaolin suspension was further selected from these 20 strains. It was identified as Bacillus sphaericus. The results of the test showed that the favorable substrates for flocculant formation were： sucrose 2 % concentration as carbon source, urea 0.15 % with peptone 0.15G as nitrogen sources. Other optimal factors of for the production of the flocculant were temperature at 30-34 ℃ ,pH of 7.0 and a growth time of 80 h. The produced flocculant could purify a wastewater of water-based ink effectively in laboratory tests with the turbidity removal of approximately 98.7 % after 15 min of flocculation.

关 键 词：微生物絮凝剂 筛选影响因素 污水处理 

分 类 号：X703.5[环境科学与工程—环境工程]