LPS对大鼠雪旺氏细胞TNF-α合成和释放的影响  

Effect of LPS on TNF-α synthesis and release in rat Schwann cells

作　　者：秦永伟[1] 沈爱国[2] 王海波[1] 邵晓轶[1] 高永静[1] 程纯[1] 

机构地区：[1]南通大学基础医学院微生物和免疫学教研室,南通226001 [2]南通大学神经再生重点实验室,南通226001

出　　处：《中国免疫学杂志》2007年第11期1004-1008,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.30300099;30500153;30770488);江苏省自然科学基金资助项目(BK2003035);江苏省高校自然科学研究项目(KJB180109;05KJB180100);江苏省社会发展科技指导性计划项目(BS2004526)资助

摘　　要：目的:探讨内毒素脂多糖(lipopolysaccharide,LPS)的诱导作用对大鼠雪旺氏细胞肿瘤坏死因子α(tumor nec-rosis factor-α,TNF-α)的表达。方法:不同浓度,不同时间用LPS刺激雪旺氏细胞,用酶联免疫吸附实验(ELISA)分别检测细胞胞液和上清中分泌的TNF-α的表达量,同时用间接免疫荧光细胞化学染色检测TNF-α的细胞定位。结果:用LPS10μg/ml刺激后2小时能显著促进雪旺氏细胞浆内TNF-α的表达,同时也检测到培养液上清中有TNF-α的释放。结论:细菌的致病因子LPS的诱导确能促进雪旺氏细胞高效表达和分泌TNF-α,从而为雪旺氏细胞作为免疫活性细胞在周围神经系统中发挥免疫调节作用提供初步依据。Objective：To explore the effects of lipopolysaccharide（LPS） on expression of TNF-α in rat Schwann cells. Methods： Schwann cells were treated with LPS at different concentration and for variant duration. TNF-α in cytoplasm and supernatant were quanti- fied by sandwich enzyme-linked immtmosorbent assay（ELISA）. Intracellular location of TNF-α was detected under confocal microscope. Results ：LPS could predominantly induce TNF-α expression in cytoplasm of rat Schwann cells, and after 10 μg/ml for 2 h,TNF-α was de- tected in the cultural supernatant. Indirect-immcnoflurescence staining indicated increased expression of TNF-α following LPS induction. Conclusion：Bacterial causative agent-LPS could induce the expression and secretion of TNF-α in rat Schwann cells, which imply that Schwann cells, as immtmocompetent cells, may play an important role in the immune regulation in the peripheral never system.

关 键 词：脂多糖 雪旺氏细胞 肿瘤坏死因子-Α 

分 类 号：R392.11[医药卫生—免疫学]