腹腔注射阿霉素建立扩张型心肌病大鼠模型的方法学改进  

Improved technology of inducing dilated cardiomyopathy animal model of rats by peritoneally injecting adriamycin

作　　者：钟国强[1] 马国添[1] 刘唐威[1] 李燕[2] 文伟明[1] 巫相宏[1] 

机构地区：[1]广西医科大学第一附属医院心内科,广西南宁530021 [2]广西医科大学第一附属医院儿科,广西南宁530021

出　　处：《基础医学与临床》2007年第11期1289-1292,共4页Basic and Clinical Medicine

基　　金：广西医疗卫生重点科研课题(重200530)

摘　　要：目的建立一种发病过程类似于人类的扩张型心肌病(DCM)大鼠模型。方法雄性SD大鼠,腹腔注射阿霉素2.8mg/(kg.week)连续11周,停药观察2周。ELISA方法检测血浆脑利钠肽(BNP)水平;超声心动图测定左室舒张末径(LVEDD)、左室收缩末径(LVESD)、左室射血分数(LVEF);观察心脏大体形态以及光学显微镜下病理改变。结果(1)DCM组BNP水平高于正常组(P<0.01);(2)DCM组LVEDD、LVESD高于正常组(P<0.01),而LVEF则低于正常组(P<0.01);(3)DCM组心脏心腔扩大,心肌细胞变性、坏死、纤维化,符合DCM的改变。结论大鼠腹腔注射阿霉素可以成功建立DCM的动物模型,该模型稳定、可靠、经济,适合研究DCM或慢性心力衰竭。Objective To develop rats model for human dilated cardiomyopathy （DCM）. Methods Male Sprague- Dawley rats were administered adriamycin intraperitoneally 2. 8 mg/kg · week） for 11 weeks, and then observed for 2 weeks. Plasma levels of brain natriuretic peptide （BNP） were studied by ELISA; left ventricular end diastolic diameter （LVEDD）, left ventricular end systolic diameter（LVESD） and left ventricular ejection fraction （LVEF） were measured by echocardiogram; and morphology of the hearts and pathological lesions of cardiac muscle tissues were observed. Results （ 1 ） The levels of BNP of the DCM group were higher than those of the normal group （P 〈0. 01 ）. （2）LVEDD and LVESD of the DCM group was longer than that of the normal group （P 〈 0. 01 ）; LVEF of the DCM group was lower than that of the normal group （P 〈 0. 01 ）. （3） The hearts of the DCM group showed that the chamber of left ventricle was enlarged; and the cardiac cells suffered from degeneration, necrosis and fibrosis, which was consistent with human DCM. Conclusion Administering adriamycin intraperitoneally successfully developed an animal model of DCM, which is stable, reliable and highly cost-effective. This model is suitable for the research of DCM and chronic heart failure.

关 键 词：阿霉素 大鼠 扩张型心肌病 动物模型 

分 类 号：R542.2[医药卫生—心血管疾病]