中医“血分论治”对寻常型银屑病患者外周血单个核细胞IFN-γ/IL-4表达影响的实验研究  

Experimental Study on Blood-Stage Treatment in Influencing Peripheral Blood Mononuclear IFN-γ/IL-4 Expression in Patients with Psoriasis Vulgaris

作　　者：李斌[1] 王婕[1] 范斌[1] 李福伦[1] 顾荻青[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院皮肤科,上海200437

出　　处：《上海中医药杂志》2007年第11期5-6,共2页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市教育委员会科研项目(30304113143);上海市重点学科建设资助项目(T0304)

摘　　要：目的从T细胞免疫角度探讨从"血分论治"寻常型银屑病的作用机制。方法选择32例寻常型银屑病患者(血热证组17例,血瘀证组15例),以10例健康人为对照,提取、体外培养外周血单一核细胞(PBMC);选取清洁级雄性Wistar大鼠,制作灌胃清热凉血方和益气活血方血清。采用实时荧光定量聚合酶链反应方法(FQ-PCR)分别观察不同中药血清刺激前后PBMC中IFN-γ/IL-4mRNA的表达变化情况。结果中药干预后,血热证(进行期)组IL-4mRNAIFN-γmRNA和血瘀证(静止期)组表达水平均明显下降(P<0.05)。结论从"血分论治"寻常型银屑病可通过调节IFN-γ/IL-4的平衡发挥治疗作用。Objective To study the action mechanisms of blood-stage treatment in the influence of psoriasis vulgaris from T cell immunity. Methods Thirty-two subjects wre observed and 10 healthy people were enrolled as control. Male Wistar rats were modeled to make serums respectively from heat-clearing and blood-cooling decoction and qi-enriching and blood-activating decoctions. Real time FQ-PCR was employed to detect the mononuclear IFN-γ/IL-4 expression. Results After herbal treatment, IFN-γ mRNA expression increased. Conclusion Blood-stage treatment works well on mononuclear IFN-γ/IL-4 expression in patients with psoriasis vulgaris.

关 键 词：寻常型银屑病 从血分论治 干扰素-γ/白介素4 实时荧光定量聚合酶链反应 

分 类 号：R275[医药卫生—中医皮科]