益气破瘀法对狼疮鼠血清IL-10与IFN-γ及肾脏病理的影响  

Effects of Qi-Enriching and Stasis-Breaking Therapy on Serum IL-10,IFN-γ and Renal Pathology in Mice

作　　者：王骁[1] 苏励[1] 曲环汝[1] 杨亚馗[1] 薛轶燕[1] 茅建春[1] 顾军花[1] 

机构地区：[1]上海中医药大学附属龙华医院风湿科,上海200032

出　　处：《上海中医药大学学报》2007年第6期51-53,共3页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30472205);上海市博士点基金资助项目(2006B09)

摘　　要：目的:观察益气破瘀法对NZB/WF1狼疮鼠的疗效,并探讨其作用机制。方法:将NZB/WF1小鼠随机分为模型组、中药组、西药组与中西医结合组。分别以生理盐水、益气破瘀小复方和骁悉对其进行干预治疗。以双抗夹心ELISA法检测血清IL-10、IFN-γ水平,肾组织行常规HE染色。结果:中药组、西药组、中西医结合组与模型组相比,血清IL-10、IFN-γ均有显著降低(P<0.01);各治疗组之间比较未见差异显著(P>0.05)。HE染色显示,中药组、西药组、中西医结合组肾脏病变与模型组相比,系膜细胞增生、系膜基质增宽等情况较轻,内皮下免疫复合物沉积较少,其中以中西医结合组病变最轻。结论:益气破瘀法能明显降低狼疮鼠血清IL-10、IFN-γ水平,延缓肾脏损害。采用中西医结合治疗的效果优于单用中药和单用西药。Objective： To investigate the therapeutic effects of qi-enriching and stasis-breaking therapy on NZB/W F1 mice, and to approach its mechanism. Methods： NZB/W F1 mice were divided into model group, herb decoction group, Cellcept group and herb-Celleept group, and were given normal saline, qi-enriching and stasis-breaking decoction and Cellcept solution respectively every day. Two months later, serum IL-10 and IFN-γ were determined by double-antibody sandwich ELISA. Kidney slices of these mice were stained by reollar HE methods. Results： The serum IL-10 and IFN-γ levels in herb decoction group, Cellcept group and herb-Cellcept group were significantly lower than those of model group, P〈0.01. But no differ- ence was found among the three different therapeutic groups. HE slice showed proliferation of mesenterium cells and matrix in the four therapeutic groups which was milder than model group, especially in herb-Cellcept group. Conclusion： Qi-enriching and stasis-breaking decoction can down-regulate the levels of serum IL-10 and IFN-γ of NZB/W FI mice and relieve their renal lesion. Therapeutic effect of herb-Cellcept is superior to herb decoction or Cellcept.

关 键 词：益气破瘀法 红斑狼疮 白细胞介素-10 干扰素-Γ 

分 类 号：R285.5[医药卫生—中药学]