不同浓度共聚物在保护超声介导细胞损伤和基因转染中的作用  

Pluronic Block Copolymers:The Functions in Protection Against Ultrasound Induced Cell Damage and Gene Delivery at Different Concentrations

作　　者：陈云超[1] 张青萍[1] 黄道中[1] 李开艳[1] 王志辉[1] 洪恺[1] 王芬[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《化学与生物工程》2007年第8期33-37,共5页Chemistry & Bioengineering

基　　金：国家自然科学基金面上项目(30670620);国家留学回国人员科研启动基金资助项目(教外司留[2005]383号)

摘　　要：选用三种不同的细胞系C2C12、3T3-MDEI和CHO-E为研究对象,考察了三种不同水溶性的共聚物F127、L61和P85在超声介导细胞损伤和基因转染中的作用。结果显示,在较低浓度时,共聚物提高了超声介导的细胞损伤(P<0.05)和基因转染(P<0.01);在较高浓度时,F127和P85可以保护超声介导的细胞损伤作用(P<0.01),而L61有较高的毒性。To investigate the functions of Pluronics block copolymers in ultrasound induced cell damage and gene delivery,three different Pluronics block copolymers F127, L61 and P85 at different concentrations were studied in three cell lines （C2C12,3T3-MDEI,and CHO-E）. The results indicated that the ultrasound could induce cell damage and the viability declined as increasing ultrasound power. At low concentration around CMC, the Pluronics block copolymers could enhance ultrasound mediated cell damage（P〈0.05）and gene delivery （P 〈0.01）. But the Pluronics block copolymers behaved differently at high concentration above 10 to 20 folds of CMC,some of which,like F127 and P85,could protect against ultrasound induced cell damage even when the power of the ultrasound was increased 10-folds,while the others,like L61 ,showed high toxicity. At the same time,they could not enhance ultrasound induced gene delivery at high concentrations. In short,Pluronics block copolymers could enhance ultrasound mediated gene delivery at low concentrations and some of which could protect against ultrasound mediated cell damage at high concentrations.

关 键 词：共聚物 超声 细胞保护 细胞损伤 基因转染 

分 类 号：R392.28[医药卫生—免疫学] R394[医药卫生—基础医学]