流动注射化学发光法测定人体血清中的胆固醇  

Determination of cholesterol in human serum by flow injection chemiluminescence method

作　　者：汪敬武[1] 吴东梅[1] 杨佳 

机构地区：[1]南昌大学化学系,江西南昌330031 [2]南昌特康科技公司,江西南昌330096

出　　处：《化学研究与应用》2007年第7期791-794,共4页Chemical Research and Application

摘　　要：Hemoglobin was used as a mimetic peroxidase in the catalytic chemiluminescence reaction of lumino and hydrogen peroxide. The hemoglobin-catalyzed reaction was coupled with the oxidation reaction of cholesterol catalyzed by COA(cholesterol oxydase , COA.) to determine total cholesterol(TC)and free cholesterol (FC) in human serum by flow injection chemiluminescence method. The sampling frequency was 30 s/h. The linear range for free cholesterol is 5.6×10-7mol/L～3.8×10-5mol/L and the detection limit is 3.0×10-7mol/L. The linear range for total cholesterol is 1.1×10-6mol/L～4.3×10-5mol/L and the detection limit is 1.5×10-7mol/L.Hemoglobin was used as a mimetic peroxidase in the catalytic chemiluminescence reaction of lumino and hydrogen peroxide. The hemoglobin - catalyzed reaction was coupled with the oxidation reaction of cholesterol catalyzed by COA （ cholesterol oxydase , COA. ） to determine total cholesterol（TC） and free cholesterol （FC） in human serum by flow injection chemiluminescence method. The sampling frequency was 30 s/h. The linear range for free cholesterol is 5. 6 × 10^-7 mol/L -3. 8 × 10^-5mol/L and the detection limit is 3.0 × 10^-7 mol/L. The linear range for total cholesterol is 1.1 × 10^-6 mol/L - 4.3 × 10^-5 mol/L and the detection limit is 1.5 × 10^-7 mol/L.

关 键 词：流动注射 血红蛋白 化学发光 游离胆固醇 总胆固醇 

分 类 号：O657.3[理学—分析化学]