绵羊肌肉生长抑制素基因外显子Ⅰ单核苷酸多态性分析  

Analysis on Mononucleotide Polymorphism of Exon Ⅰ of Myostatin Gene in Sheep

作　　者：张慧玲[1] 史洪才[1] 罗淑萍[2] 牛志刚[1] 唐森[1] 武坚[1] 刘明军[1] 

机构地区：[1]新疆畜牧科学院农业部家畜繁育生物技术重点实验室 [2]新疆农业大学农学院,乌鲁木齐830052

出　　处：《新疆农业大学学报》2007年第4期21-24,共4页Journal of Xinjiang Agricultural University

基　　金：新疆维吾尔自治区自治区高技术研究发展计划项目(200611106)

摘　　要：利用PCR-SSCP和DNA测序法,检测了德国肉用美利奴、特克塞尔、多浪羊和陶塞特等4个绵羊品种肌肉生长抑制素基因外显子Ⅰ的单核苷酸多态性。研究结果表明:在德国肉用美利奴肌肉生长抑制素基因外显子Ⅰ第+84位(相对翻译起始位点ATG)发现G→A的突变,但仅存在两种基因型,即GG和GA,基因型频率分别为0.6944和0.3056。该突变位点编码的氨基酸序列不发生改变,在其余样品均未检测到多态。Myostatin is a secreted protein that acts as a negative regulator of skeletal muscle mass. In this study,PCR-SSCP and DNA sequences were used to detect the polymorphism of mononucleotide within MSTN exon I in different sheep breeds,including German Merino, Texel,Doxset and Duolang sheep. As a result,a G--~A mutation was identified at ^-84（relative to translational initiation site） in German Merino. Two genotypes were found,and the genotype frequencies for GG and GA were 0. 6944 and 0. 3056. The mutation is a synonymous mutation and polymorphism had not been detected in other breeds.

关 键 词：绵羊 肌肉生长抑制素基因 单核苷酸多态性 

分 类 号：S826[农业科学—畜牧学] Q523.8[农业科学—畜牧兽医]