力竭游泳运动对大鼠心肌细胞中间纤维和超微结构的影响  

The effects of intermediate filaments and ultrastructure of rat′s cardiac muscle by single exhausted swimming

作　　者：王鑫 陈正东 吴昊 吴洪海 朱永泽 

机构地区：[1]江苏省扬州五台山医院,扬州市新民路225003 [2]扬州大学

出　　处：《中国康复医学杂志》2007年第11期968-970,973,I0002,共5页Chinese Journal of Rehabilitation Medicine

基　　金：江苏省教育厅自然科学基金资助项目[05KJD310267]

摘　　要：目的:探讨力竭游泳运动对大鼠心肌细胞中间纤维和超微结构的影响,以期为运动对心肌的生理和病理变化研究提供形态学依据。方法:SD大鼠进行1次力竭游泳,分别在力竭游泳运动后即刻(0h)、3h、24h、48h和100h,取左心室肌进行石蜡切片样本处理和常规透射电镜样本处理,石蜡切片分别进行结蛋白和波形蛋白的免疫组织化学染色(Envision法),观察和计算其平均光密度和阳性面积,电镜标本观察肌细胞内超微结构的改变。结果:1次力竭游泳后从即刻到24h,心肌结蛋白和波形蛋白持续降低至最低点,超微结构也出现损伤性改变;运动后48h,心肌结蛋白和波形蛋白开始增加,超微结构的损伤也开始改善。结论:力竭游泳运动可以引起大鼠心肌中间纤维和超微结构的形态变化,运动后大鼠心肌细胞的中间纤维和超微结构出现逐渐恢复的过程。Objective： To explore the effects of intermediate filaments（IF） and ultrastructure of rat＇s cardiac muscles by single exhausted swimming （SES）, so as to provide some morphological basis of the changes of physiology and pathology by the exercise-induced injury and recuperation of cardiac muscle. Method： After exercises of SES, rat＇s cardiac muscles were cut off on Oh, 3h, 24h, 48h and 10Oh. These specimens were treated respectively with regular procedures of paraffin sections and transmission electron microscope（TEM）. The paraffin sections were stained by Desmin and Vimentin immunohistochemistry,their mean optics density and percentage of stained areas were observed and calculated. The uhrastructure of muscles were observed by TEM. Result ＆ Conclusion： Single exhausted swimming exercises will provide effects on IF and ultrastrueture of rat＇s cardiac muscles, and the changes of IF and ultrastructure of cardiac muscle cell after exercises of SES are a gradual recuperation process.

关 键 词：大鼠 游泳 心肌 结蛋白 波形蛋白 超微结构 

分 类 号：R87[医药卫生—运动医学]