正己烷中毒大鼠角膜神经组织损伤的研究  

Morphological changes of corneal nerve in rats exposed to n-hexane

作　　者：陈瑛[1] 程欣[2] 唐福星[3] 刘阳[4] 徐锦堂[5] 

机构地区：[1]广州市第十二人民医院眼科,510620 [2]暨南大学医学院组胚教研室 [3]广州市第十二人民医院电镜室,510620 [4]广州市第十二人民医院外科,510620 [5]广州市第十二人民医院眼科研究室,510620

出　　处：《中华劳动卫生职业病杂志》2007年第11期667-670,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广东省科技计划项目(2006B36006015);广东省医学科研基金(A2006566)

摘　　要：目的研究正己烷染毒大鼠角膜神经和角膜组织的损伤。方法将32只雄性SD大鼠随机分成对照组和正己烷染毒组。采取静式吸入染毒方式,正己烷浓度为35.2g/m^3,染毒1、3、7、14d后处死大鼠,对照组为吸入空气的空白对照。采用氯化金染色和透射电镜观察正己烷对角膜神经的损害,同时观察角膜组织病理学和超微结构的改变。结果染毒各组大鼠全血中正己烷的平均浓度分别为(242.91±59.68)、(668.77±221.74)、(1021.21±545.71)、(1140.42±468.44)μg/L,随染毒时间延长呈梯度上升。正己烷染毒7和14d,大鼠角膜神经纤维稀疏、变细,中央神经网密度下降;电子显微镜下可见,角膜上皮内神经末梢轴膜不完整,轴浆部分溶解或空泡样改变,轴浆内微管和小泡减少。染毒14d,大鼠角膜上皮细胞微绒毛减少,部分基底细胞出现核固缩或核内空泡,线粒体水肿或消失。结论正己烷可造成大鼠角膜神经和角膜组织的明显损害,将导致角膜功能异常。Objective To investigate the injury in the corneal nerve and cornea of rots exposed to n-hexane. Methods Thirty-two SD male rats were randomly divided into one control group and four n-hexane groups. The four n-hexane groups inhaled 35.2 g/m^3 n-hexane statically for 1, 3, 7 and 14 d respectively, while the rats in the control group inhaled air. The corneal nerve damage was investigated with golden staining and transmission electron microscope. Histopathological and uhrastructure changes of cornea were analyzed also. Results The concentration of n-hexane in blood of rats in different experimental groups was （242.91±59.68）, （668.77± 221.74）, （1021.21±545.71） and （1140.42±468.44）μg/L, increased gradiently with time exposed to n-hexane. In the rats exposed to n-hexane for 7 and 14 d, there appeared fewer corneal nerve bundles and lower density of nerve fiber at the center of cornea; under electron microscope, the lamellar sheath of nerve fiber in the corneal epitheliums appeared intermittent, the neuroplasm of endings was partly lysed and became vacuolar; the micro- filament and racuole of neuraxon decreased. In the group exposed to n-hexane for 14 d, the microvillus of cornea epithelium were decreased. In some basal cells there appeared pyknotic nucleus and vacuole, mitochondria were swollen or disappeared. Conclusion The structure of corneal nerve and cornea is damaged in the rats exposed to n-hexane, thus leading to dysfunction of cornea.

关 键 词：正己烷:角膜神经 角膜 组织病理 超微结构 

分 类 号：R114[医药卫生—卫生毒理学]