中药胃肠舒对胃肠平滑肌细胞ATP生成的影响  

Effect of traditional Chinese medicine Weichangshu on ATP in gastrointestinal smooth muscle cells

作　　者：宋晓冬[1] 刘孟安[2] 孙丰润[2] 刘颖[2] 张丽霞[1] 牛新华[1] 闫苗苗[1] 王晓江[1] 

机构地区：[1]滨州医学院基础学院 [2]滨州医学院临床学院,山东省滨州市256603

出　　处：《世界华人消化杂志》2007年第30期3159-3162,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No.30672680~~

摘　　要：目的:研究胃肠舒对胃肠平滑肌细胞动能的影响.方法:胃肠平滑肌细胞离体培养,分为5组:空白对照组、西沙必利组(0.51 g/L)、胃肠舒Ⅰ组(25 g/L)、胃肠舒Ⅱ组(50 g/L)、胃肠舒Ⅲ组(75 g/L),采用Celltiter-GloTM法和Rhodamine fluorescence法分别检测胃肠舒对胃肠平滑肌细胞ATP和线粒体跨膜电位的的影响.结果:胃肠舒作用24h后与对照组和西沙必利组相比能显著增高平滑肌细胞ATP的含量.随着胃肠舒用药浓度的增加改变ATP的含量也加大(P<0.05).流式细胞仪结果表明,胃肠舒明显加大了胃肠平滑肌细胞的线粒体跨膜电位,并且随着胃肠舒用药浓度的增加,跨膜电位的增加也越大.结论:胃肠舒可能通过改变胃肠平滑肌细胞的动能促进胃肠运动.AIM： To study the effect of Weichangshu on the kinetic energy of gastrointestinal smooth muscle cells. METHODS： Gastrointestinal smooth muscle cells were divided into five groups： control, cultured in normal medium; cisapride, cultured in normal medium with 0.51 g/L cisapride; Weichangshu Ⅰ, cultured in normal medium with 25 g/L Weichangshu; Weichangshu Ⅱ, cultured in normal medium with 50 g/L Weichangshu; and Weichangshu Ill, cultured in normal medium with 75 g/L Weichangshu. Celltiter-Glo was used to detect ATP. The relative changes in mitochondrial potential were tested with the rhodamine fluorescence （R-123） technique.RESULTS： After exposure to Weichangshu for 24 hours, the change in ATP content was more significant （P 〈 0.05） and the intensity of R-123 fluorescence in gastrointestinal smooth muscle cells was significantly increased compared with those in the control and cisapride groups. As the Weichangshu concentration increased, so did the content of ATP and mitochondrial potential. CONCLUSION： Weichangshu may change the kinetic energy of gastrointestinal smooth muscle cells, leading to an improvement in gastrointestinal motility.

关 键 词：中药 胃肠舒 ATP 线粒体跨膜电位 Celltiter-GloTM法 RHODAMINE fluorescence法 

分 类 号：R285.5[医药卫生—中药学]