乳癌患者^(99m)Tc-MIBI显像及癌组织中P-糖蛋白、多药耐药相关蛋白测定  

^(99m)Tc-MIBI imaging of breast cancer and its expression of p-glycoprotein and multidrug resistence-associated protein

作　　者：韩星敏[1] 刘保平[1] 程兵[1] 谢新立[1] 阮翘[1] 孙秉奇[1] 林开文 

机构地区：[1]郑州大学第一附属医院核医学科,郑州450052 [2]许昌市中心医院核医学科,许昌461000

出　　处：《郑州大学学报（医学版）》2007年第6期1133-1136,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:评价乳癌患者99mTc-甲氧基异丁基异腈(MIBI)显像结果,并检测癌组织中P-糖蛋白(P-gp)、多药耐药相关蛋白(MRP)的表达。方法:42例乳腺癌患者静脉注射99mTc-MIBI555~740MBq(15~20mCi),10min及3h后显像。计算早、晚期肿瘤部位平均放射性计数与对侧相应部位平均放射性计数比值(T/N)、清除率。术后标本采用免疫组织化学法检测P-gp、MRP的表达。结果:根据P-gp、MRP免疫组织化学结果将42例患者分为4组:A组P-gp阳性、MRP阳性,7例;B组P-gp阳性、MRP阴性,15例;C组P-gp阴性、MRP阳性,9例;D组P-gp阴性、MRP阴性,11例。早期T/N值,A组、B组与D组比较,差异均有统计学意义(P均<0.05),其余各组间差异无统计学意义(P均>0.05)。晚期T/N值,各组间差异无统计学意义(F=0.460,P=0.650)。清除率,A组与B组、C组、D组之间,B组、C组与D组之间,差异均有统计学意义(P均<0.05),而B组与C组之间,差异无统计学意义(P>0.05)。结论:乳癌99mTc-MIBI显像计算清除率可用于评估癌组织中P-gp、MRP的表达。Aim ： To evaluate the relationship between the result of ^99mTc-MIBI imaging of breast cancer and the expression of p-glycoprotein（P-gp） and multidrug resistenee-assoeiated protein（MRP）. M ethods：A total of 42 patients with breast cancer were given 555 - 740 MBq （ 15 - 20 mCi） ^99mTc-MIBI intravenously. Breast imaging was performed 10 min （ early phase） and 3 h （ delayed phase） after the injection. The tumor-to-normal （T/N） ratio and wash-out rate （WR） were calculated on both early and delayed images. The expressions of P-gp and MRP in breast cancer tissues were examined by immunohistoehemistry. Results：The 42 patients were divided into 4 groups according to the results of immunohistochemistry： group A, P-gp（＋） and MRP（＋）, 7 patients;group B, P-gp（＋） and MRP（-）, 15 patients;group C, P-gp （ - ） and MRP（ ＋ ） , 9 patients;group D, P-gp（ - ） and MRP（ - ） , 11 patients. The early T/N ratio was significant lower in group D than that in group A and B （ P 〈 0.05）. The delayed T/N ratio had no significant difference among the 4 groups （ F = 0. 460, P = 0. 650）. There were significant differences on WR between group A and B, group A and C, group A and D, group B and D, group C and D （ P 〈 0.05）. However, no significant difference was found between group B and C （P 〉 0.05）. Gonclusion： WR calculated from ^99mTc-MIBI imaging of breast cancer is useful for determining expression of P-gp and MRP.

关 键 词：乳癌 ^99MTC-甲氧基异丁基异腈 P-糖蛋白 多药耐药相关蛋白 

分 类 号：R817.4[医药卫生—影像医学与核医学]