乙型肝炎病毒C基因启动子变异与白细胞介素10与12、肿瘤坏死因子α、γ-干扰素及病毒含量的关系  

Correlation of Hepatitis B Virus Basic Promoter Mutation with IL-10,IL-12,TNF-α,IFN-γ as well as HBV DNA Contents in Chronic Hepatitis

作　　者：黄力毅[1] 玉艳红[1] 宣伟军[2] 吴继周[1] 黄璐[1] 

机构地区：[1]广西医科大学第一附属医院,广西南宁530021 [2]广西中医学院,广西南宁530001

出　　处：《中华医院感染学杂志》2007年第11期1321-1324,共4页Chinese Journal of Nosocomiology

基　　金：广西科学基金项目(桂科自0339050);广西卫生厅课题(桂卫Z2004127)

摘　　要：目的探讨慢性肝病患者乙型肝炎病毒C基因启动子变异(HBV BCP)与血清白细胞介素10(IL-10)、12(IL-12)、肿瘤坏死因子α(TNF-α)、γ-干扰素(IFN-γ)及病毒含量(HBV DNA)的关系。方法采用PCR微板核酸杂交结合ELISA检测显示技术,对176例HBV慢性感染者(慢性乙型病毒性肝炎轻、中、重度,肝炎肝硬化,慢性重型肝炎和原发性肝癌)血清进行检测HBV BCP区核苷酸(nt)1762碱基A→T和1764碱基G→A联合突变;采用双抗体夹心ELISA检测技术,检测患者血清细胞因子(IL-10、IL-12、TNF-α和IFN-γ)水平。结果HBVBCP变异阳性组、阴性组间对细胞因子(IL-10、IL-12、TNF-α和IFN-γ)及HBV DNA复制水平的影响差异有统计学意义(P<0.05),BCP变异阳性组的血清IL-10(80.96±30.86vs72.11±24.19 mg/L)I、L-12(41.33±15.10vs35.98±14.47 mg/L)、TNF-α(56.04±27.05vs38.01±10.49 mg/L)、IFN-γ(19.81±12.29vs16.55±8.99mg/L)和HBV DNA(108.2478±0.9826vs105.8876±1.4822拷贝/ml)的水平明显高于BCP变异阴性组。结论HBV BCP变异可导致血清IL-10I、L-12、TNF-α、IFN-γ和病毒复制水平增高。OBJECTIVE To study the correlation of hepalitis B virus （HBV）basic core promoter （BCP） mutation with the interleukins （IL-10,IL-12）, tumor necrosis factor （TNF）-α, interferon （IFN）-γ, as well as HBV DNA contents in patients with chronic hepatitis B virus infection. METHODS （1） Project subject： 176 patients [chronic hepatitis B with mild, moderate and severe degree; liver cirrhosis, chronic fulminant and hepatocellular carcinoma （HCC）] with chronic hepatitis B virus infection were studied. （2） Project methods： （I） The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1734 were determined by the method of polymerase chain reaction （PCR） microplate hybridization ELISA in these patients. ② The serum cytokines（IL-10, IL-12, TNF-α and IFN-γ） of these patients were measured by specific-ELISA. RESULTS The serum levels of cytokinesCIL-10 （80.96ff=30.86 vs 72.11±24.19 mg/L）, IL-12（41.33±15.10 vs 35.98±14.47 mg/L）, TNF-α（56.04ff=27.05 vs 38.01 ±=10.49 mg/L）, IFN-γ（19.81±12.29 vs 16. 155±8.99 mg/L）and HBV DNA contents （108. 2478 ± 0. 9826 vs 105. 8876±1. 4822copies/ml） in BCP mutant group were significantly higher than that in non-mutant group （P〈0.05）. CONCLUSIONS The mutants in HBV BCP might enhance the serum levels of cytokines （IL-10, IL-12, TNF-a and IFN-α）, which could lead to the replication of HBV DNA.

关 键 词：乙型肝炎病毒 核心基因启动子 基因变异 白细胞介素10 白细胞介素12 肿瘤坏死因子Α γ-干扰 素 

分 类 号：R512.62[医药卫生—内科学]