龙葵碱对HepG2细胞内[Ca^(2+)]i的影响  

Effects of solanine on [Ca^(2+)]i in HepG2 cell

作　　者：高世勇[1] 邹翔[1] 王宏亮[1] 季宇彬[1] 

机构地区：[1]哈尔滨商业大学生命与环境研究中心药物研究所博士后科研工作站,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2007年第5期513-516,519,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金(30400591);黑龙江省自然科学基金(D200505;D-2004-13);黑龙江省教育厅骨干教师创新能力资助计划(2006);哈尔滨市青年基金(2004AFQXJ035)

摘　　要：探讨龙葵碱对HepG2细胞形态及细胞内[Ca^(2+)]i的影响,揭示龙葵碱诱导细胞凋亡的机制.以人肝癌细胞HepG2为研究对象,采用AO/EB双染HepG2细胞,激光共聚焦扫描显微镜观察细胞形态学改变,Fluo-3/AM单染HepG2细胞,激光共聚焦扫描显微镜观察细胞内[Ca^(2+)]i的改变.结果发现龙葵碱作用于HepG2 48h后,细胞形态出现典型的细胞凋亡形态;细胞内[Ca^(2+)]i浓度明显升高.表明龙葵碱升高细胞内Ca^(2+)浓度启动细胞凋亡机制.To study the effects of solanine on morphology and [ Ca^2＋ ] i in cultural cancer cell line HepG2. HepG2 cell is treated with solanine at serial concerntration of 2,0. 4,0. 08, 0. 016 , 0. 0032 μg/mL. The morphology is analyzed by using confocal with AO/EB labeling method. The same method is adopted to detect the [ Ca^2＋ ] i with Fluo - 3/AM labeling. The results show that the morphology of cell apoptosis is appear and the Ca^2＋ concentration is increased in HepG2 cell after treating with solanine for 48 h. Therefore, solanine induces the HepG2 cell apoptosis by increasing the Ca^2＋ of cytoplasm in HepG2.

关 键 词：龙葵碱 HEPG2细胞 形态学 细胞内CA^2+ 激光共聚焦扫描术 

分 类 号：R285[医药卫生—中药学]