干旱胁迫下水稻叶片光合速率与叶绿素含量的相关性及其基因定位  

Correlation Analysis and Gene Identification for Chlorophyll Content and Photosynthetic Rate in Rice Leaves under Drought Stress

作　　者：胡颂平[1] 王正功[1] 张琳[1] 刘国兰[2] 罗利军[2] 廖慧敏[1] 

机构地区：[1]吉首大学资源与环境学院,湖南省吉首市416000 [2]上海市农业生物基因中心,上海201106

出　　处：《中国生物化学与分子生物学报》2007年第11期926-932,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家重点基础研究发展规划(973计划;No.2003AA207010)项目;国家高技术研究发展计划资助项目(863计划;No.2004B17200);洛克菲勒基金;吉首大学校人事基金资助~~

摘　　要：以水稻重组自交系珍汕97B×IRAT109 F9代群体195个株系为材料,用213个简单重复系列(SSR)标记构建了基于该群体的连锁图谱,对水稻叶片叶绿素含量和光合速率在干旱和正常条件下的数量性状位点(QTL)和双基因互作进行了分析,同时分析了叶绿素含量与光合速率的相关关系.结果表明:叶绿素含量与光合速率在正常供水下呈极显著正相关(r=0.1857,表示在1%水平上显著),但在干旱下则表现无关(r=0.0766).控制叶绿素含量的基因很复杂,主效QTL有13个,位于1、2、3、4、5、6、10号染色体上;其中,在干旱处理下检测到的主效QTL有6个,位于1、2、3、4、5号染色体上;在正常供水下检测到的主效QTL有7个,位于2、3、4、6、10号染色体上.在干旱和正常条件下它们分别解释了47.39%和56.19%的表型变异;在2种处理下均检出的主效QTL是2、3、4号染色体上的qCC2a、qCC2b、qCC3a、qCC3c、qCC4a、qCC4b;它们位于同一染色体的相同区段.在干旱和正常条件下检测到4个QTL与光合速率有关;其中干旱下有3个(qPR2、qPR10、qPR11),正常条件下1个(qPR10).它们分别被定位于2、10、11号染色体,共解释13.94%的表型变异.叶绿素含量互作效应位点有16对,涉及除10号染色体外的所有染色体;干旱下,有4对互作基因,共解释18.57%的表型变异,分别位于1-7、2-4、5-8、6-12号染色体上;正常供水下,有12对互作基因,共解释38.49%的表型变异,分别位于1-3、1-4、1-8、2-4、2-5、3-5、4-11、4-12、5-9、7-12、8-11号染色体上,其中3-5号染色体不同区段上有两对互作效应位点.The relationships of chlorophyll content （ CC ） and photosynthetic rate （ PR ） of rice leaves and their main-QTLs were studied by using a population of recombinant inbred lines （RILs） （Zhenshan97B ×IRAT109 ） in water gradient condition. There was significant correlation between CC and PR （R = 0. 185 7 ） in well watered condition, but no correlation in drought stress （ r = 0.076 6）. A total of 13 main effects of QTLs locating on chromosomes 1,2, 3,4, 5,6 and 10 were found to associate with CC, which included six QTLs locating on chromosomes 1,2, 3,4, 5 in drought stress, and seven QTLs locating on chromosomes 2,3,4,6, 10 in normal condition. They explained 47.39% and 56.19% phenotypic variation, respectively, in drought and normal conditions. Six QTLs of them （qCC2α, qCC2b, qCC3α, qCC3c, qCC 4α, qCC4b） detected in both conditions were mapped on chromosomes 2,3 and 4. In the same time, 4 main effects of QTLs related to PR were located on chromosomes 2, 10 and 11 respectively with accounting total 13.94% phenotypic variation. They included three QTLs （qPR2, qPR10, qPR11） in drought stress and one QTL in normal （qPR10）. A total of 16 pairs of epistatic QTLs of CC were detected on all chromosomes except chromosome 10. Four pairs of them were found in drought stress with explaining total 18.57% phenotypic variation and twelve pairs of them were detected in well watered condition with accounting total 38.49% phenotypic variation.

关 键 词：抗旱性 光合速率 叶绿素含量 QTL分析 水稻 

分 类 号：Q754[生物学—分子生物学] Q343.17