用激光拉曼光谱区分胃癌变细胞与正常细胞  

Differentiating Gastric Cancer Cell from Normal Cell by Laser Raman Spectrum

作　　者：黄鹰[1] 陶家友[1] 蔺蓉[2] 李毅[1] 侯晓华[2] 易新建[1] 

机构地区：[1]华中科技大学光电子科学与工程学院,湖北武汉430074 [2]华中科技大学同济医学院附属协和医院消化科,湖北武汉430022

出　　处：《光谱学与光谱分析》2007年第11期2262-2265,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60477040);湖北省自然科学基金项目(2004ABA217)资助

摘　　要：利用激光拉曼光谱对胃癌细胞及正常胃细胞进行了对比检测,对胃癌细胞特征拉曼峰作了初步探讨,发现胃癌患者标本的特征拉曼峰与非胃癌患者标本的光谱有明显的不同。实验发现,浓度为每mL1.25×105个胃癌细胞的样品在经过若干天培养后均能够被检测出胃癌细胞的特征拉曼峰,分别位于特定波长583,633,656nm处。这是几个很少见诸报道的新拉曼光谱峰。不论是特征拉曼峰,还是在胃癌细胞及正常胃细胞的几个共有的拉曼峰674,707,773,799nm处,拉曼光谱强度都是随血清中癌细胞浓度的增加而增加。激光拉曼光谱分析有可能发展成为一种快速有效的癌细胞检测方法。The gastric cancer cell and normal cell were comparatively detected by means of laser Raman spectrum in the present paper. The special Raman spectrum wave crest of gastric cancer cell was inquired. It was found that the special Raman spectrum wave crest of gastric cancer cell differs from the one of normal cell After the sample at concentration of 1.25×10^5 individual · mL^-1 was cultivated for several days, a few special Raman spectrum wave crests of sample at 583,633 and 656 nm respectively were detected. This is a few new special Raman spectrum wave crests rarely reported. Whether at the special Raman spectrum wave crests or at the same several Raman spectrum wave crests 674,707,773 and 799 nm for both canner cell and normal cell, the Raman spectrum intensity increases with the cancer cell concentration. As a result, the laser Raman spectrum analyse is considered truly a kind of rapid and effectual method to check cancer cell.

关 键 词：激光拉曼光谱 胃癌细胞 光电检测 

分 类 号：O657.3[理学—分析化学] R735.2[理学—化学]