血卟啉衍生物与血红蛋白相互作用的光谱特性研究  

Spectral Properties of the Hemoporphyrin Derivative Interacting with Hemoglobin

作　　者：冯尚源[1] 陈荣[2] 杨文琴[1] 李永增[2] 黄祖芳[2] 廖小华[2] 

机构地区：[1]福建师范大学化学与材料学院实验中心,福建福州350007 [2]福建师范大学医学光电科学与技术教育部重点实验室,福建福州350007

出　　处：《光谱学与光谱分析》2007年第11期2279-2282,共4页Spectroscopy and Spectral Analysis

基　　金：教育部重点项目(205081);福建省科技厅重点项目(2005I009);福建省教育厅B类项目(JB05310)资助

摘　　要：采用荧光光谱法和紫外-可见吸收光谱法研究了在不同温度下,血卟啉衍生物(HPD)与血红蛋白(Hb)结合反应的荧光猝灭光谱行为。证实由于非辐射能量转换机制,HPD对Hb有较强的荧光猝灭能力。根据Stern-Volmer方程和Lineweaver-Burk双倒数函数曲线,计算其结合常数在高温时要比低温时大,并获得相关结合常量和热力学参数。根据Fster能量传递原理计算出临界结合位点R0=3.22nm。The quenching reaction of hemoporphyrin derivative with hemoglobin （Hb） was studied by using fluorescence spectra and absorption spectra. It was shown that HpD has a powerful ability to quench the Hb fluorescence via a nonradiative energy transfer mechanism. The formation constants of them were analyzed at different temperature according to Stem-Volmer equation and double-reciprocal equation, which are bigger at high temperature than at low temperature. The critical binding site was calculated （R0 =3.22 nm） by Foster energy transfer mechanism, and the thermodynamic parameters were obtained.

关 键 词：血卟啉衍生物(HPD) 血红蛋白(Hb) 荧光光谱 猝灭 

分 类 号：O657.3[理学—分析化学]