氯氧化锆-氯化铵荧光光谱法测定葡萄糖共存下的果糖  

Fluorometric Determination of Fructose in the Presence of Glucose Using Zirconyl Chloride-Ammonium Chloride

作　　者：童蕾[1] 王焰新[1] 赵中一[2] 金继红[2] 郭小慧[2] 

机构地区：[1]中国地质大学环境学院与生物地质与环境地质教育部重点实验室,湖北武汉430074 [2]中国地质大学材料科学与化学工程学院,湖北武汉430074

出　　处：《光谱学与光谱分析》2007年第11期2313-2316,共4页Spectroscopy and Spectral Analysis

基　　金：科技部"863"计划项目(2004AA601050)资助

摘　　要：文章根据ZrOCl2水解产生的[ZrO(OH)]+能与果糖作用产生荧光的原理,建立了果糖的定量测定方法。在NH4Cl介质中,体系的荧光强度有所增强,产物在408nm处发荧光,在0.5～25μg·mL-1范围内荧光强度与果糖浓度成正比,当反应温度为70℃、反应时间15min时,葡萄糖不干扰测定。该法成功用于水果中果糖的测定,灵敏度高,检出限达0.071μg·mL-1,方法稳定性好,结果满意。According to the principle that Zn（OH）^＋ produced by ZrOCl2 upon hydrolysis in water can react to form a fluorescent derivative with fructose, a method for the quantitative determination of fructose has been proposed. The fluorescence of the derivative enhanced at 408 nm in NH4Cl medium has a direct ratio with fructose in the linear range of 0.5-25μg·mL^-1. When reaction conditions such as a temperature of 70℃and a time of 15 min are used, the detection limits is 0. 071 mg·mL^-1. The proposed method was also applied successfully to the determination of fruit, satisfaction with high accuracy and good reproduction.

关 键 词：果糖 葡萄糖 氯氧化锆-氯化铵 荧光光谱法 

分 类 号：O657.3[理学—分析化学]