原子吸收法测定椒目中不同化学形态的锌含量  

Speciation Analysis of Zinc in Bunge Pricklyash Seed by AAS

作　　者：涂剑波[1] 王四旺[1] 谢艳华[1] 李丁川[2] 高双斌[1] 王剑波[1] 

机构地区：[1]第四军医大学药学系药物研究所,陕西西安710032 [2]解放军305医院,北京100017

出　　处：《光谱学与光谱分析》2007年第11期2357-2360,共4页Spectroscopy and Spectral Analysis

基　　金：陕西省中医药管理局项目(2005079)资助

摘　　要：结合椒目的市场前景与药效,采用原子吸收分光光度法,分析了不同产地椒目中的锌含量及其形态。使用0.45μm滤膜、CHCL3将椒目水煎液中的锌分为悬浮态和可溶态、有机态和无机态,建立了锌的四种形态分离分析方法;探讨了正辛醇-水分配体系模拟水煎液中锌在人体胃肠中的分配情况;并采用原子吸收分光光度法测定了椒目中的锌在不同溶剂中的化学形态和溶出特性。结果表明,椒目中的锌以水可煎出态为主,不同产地椒目锌含量不同,但水可煎出态总锌百分率、有机态总锌百分率基本接近。该方法回收率在97.5%～104.0%范围内,相对标准偏差均小于2.36%。应用于实际,结果表明其灵敏度高,重现性好。A method was developed for the investigation of the contents and the speciation of zinc in bunge pricklyash seed （BPS） in different habitats by atomic absorption spectrum （AAS）. By using 0. 45μm filter membrane and chloroform, four different species of the decoction of BPS, named the suspended, the soluble, the inorganic and organic species, were obtained, and the segregation analysis methods for those species were developed. The octanol-water system was proposed to simulate the distribution of decocted zinc in the stomach and the intestine of the human body, The results showed that the decocted zinc was the main content o~ the total zinc, and the contents of zinc were different in different habitats, but the percentages of decocted zinc and the organism zinc were similar. The recovery for the method was 97.5%-104. 0% and the relative standard deviation was less than 2. 36%. The results showed that the sensitivity and reproducibility of this method were excellent.

关 键 词：原子吸收光谱 形态分析 锌 椒目 

分 类 号：O657.3[理学—分析化学]