钩藤总碱与夏天无总碱合用对大鼠脑缺血再灌注的影响  

Effects of Combined Use of Total Alkaloids of Uncaria Rhynchophylla and Coryadlis Ambailis Migo on Cerebral Ischemia-reperfusion Injury in Rats

作　　者：胡雪勇[1] 孙安盛[1] 眭玉霞[2] 吴芹[1] 余丽梅[1] 黄燮南[1] 石京山[1] 

机构地区：[1]遵义医学院药理学教研室 [2]福建省立医院药剂科

出　　处：《中国中西医结合杂志》2007年第11期1007-1009,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：贵州省科技厅资助课题(No.2003040)

摘　　要：目的观察钩藤总碱(钩藤)与夏天无总碱(夏天无)合用对脑缺血/再灌注损伤的保护作用。方法各组造模前灌胃给药每天2次,共5次,末次给药30min后,采用大鼠大脑中动脉缺血(2h)/再灌注(22h)制作脑缺血损伤模型,造模12h后再给药1次,并进行神经病学评分,检测脑梗塞范围及脑组织水含量,大鼠脑组织中一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的变化。结果两药合用可降低大鼠脑缺血/再灌注后神经病学评分及梗塞范围,降低脑组织MDA含量、NOS活性及升高SOD活性(P<0.05),联合用药的效应较单用钩藤或夏天无增强(P<0.05),而对中枢抑制作用较单用钩藤减轻。结论钩藤和夏天无联合应用对脑缺血/再灌注损伤有保护作用,其作用机制与抑制脑NOS活性、提高SOD活性及减少脑神经细胞脂质过氧化损伤有关。Objective To study the effects of combined use of total alkaloids （TA） of Uncaria rhyn- chophylla （UR） and Coryadlis ambailis migo （CAM） on cerebral ischemia/reperfusion injury in rats. Methods Rat model of middle cerebral artery ischemia/reperfusion was established, the changes of neurological state was scored before and after treatment with the two kinds of TA, single or combined, and the changes of cerebral in- farcted volume, cerebral water content, activities of NOS and SOD and content of MDA in rats＇ brain were esti- mated as well. Results After being treated with the combination of both TA, the average neurological score, cerebral infracted volume, cerebral water content, activity of NOS and content of MDA in the model rats signifi- cantly decreased, and the activity of SOD was significantly increased （all P 〈 0.05）. The effect of combined use of the two TA was higher than that of use TA of UR or CAM alone （P 〈 0.05）. Moreover, the central nervous system inhibitory effect induced by combined TA was significantly weaker than that of UR. Conclusion Combined use of TA of UR and CAM may facilitate the protection against cerebral ischemia/reperfusion dam- age, the action mechanism might be relevant to reducing the lipid peroxidation injury of brain cells through in- hibiting the NOS activity and increasing the SOD activity.

关 键 词：钩藤总碱 夏天无总碱 脑缺血/再灌注 神经病学评分 

分 类 号：R285[医药卫生—中药学]