四季青药材HPLC指纹图谱研究  

Study on Fingerprinting of Folium Ilicis Purpurea by HPLC

作　　者：屈巧玲[1] 杨滨[1] 王谦鹏[2] 易崇勤[2] 肖永庆[1] 王永炎[3] 

机构地区：[1]中国中医科学院中药研究所 [2]太极集团北京产品设计中心,北京100054 [3]中国中医科学院,北京100700

出　　处：《中国药学杂志》2007年第22期1699-1703,共5页Chinese Pharmaceutical Journal

基　　金：博士后科学基金(2004035072)

摘　　要：目的建立四季青药材的HPLC指纹图谱,为该药材的质量评价提供实验依据。方法采用高效液相色谱技术,ZorbaxSbC18柱(4.6mm×250mm,5μm),1%冰醋酸(含0.2%三乙胺)-乙腈梯度洗脱,检测波长为280nm,柱温为30℃,流速为1.0mL·min-1。结果指纹图谱研究和含量测定方法学考察的各项参数均符合有关规定,以系统聚类分析将样品分为两类,每类中各批样品的相似度均大于0.85。结论HPLC指纹图谱的建立为四季青药材的质量评价提供了实验依据。OBJECTIVE To establish a HPLC-FP for the determination of Folium Ilicis Purpurea. METHODS HPLC analysis was performed on a Zorbax Sb C18 column （4.6 mm×250 mm,5 μm） ,the mobile phase used in gradient elution consisted of 1% acetic acid solution （including 0. 2% triethylamine） and acetonitrile, with the flow rate of 1.0 mL·min^-1. The column temperature was 30 ℃ and the wavelength was 280 nm. RESULTS 14 Common peaks were obtained in 20 batches of samples. The samples were divided into two groups by hierarchical clustering analyses. The similarity of each fingerprint within one group was higher than 0. 85. CONCLUSION The established method can be used for quality control of Folium Ilicis Purpurea.

关 键 词：四季青 高效液相色谱指纹图谱 含量测定 

分 类 号：R282[医药卫生—中药学]